Abstract
Purpose :
The growth characteristics of clinical corneal isolated A. fumigatus cultivated in different nutrition conditions in vitro were investigated and compared with that of standard A. fumigatus strain in order to find out the key pathogenicity of clinical pathogen.
Methods :
Five kinds of media with different glucose and peptone concentrations were prepared in both liquid and solid form. Clinical corneal isolate A. fumigatus strain and the standard strain were cultivated in the media. They were all incubated at 29 degree centigrade for 96h and observed at defined time points. The optical density was recorded to generate the growth curves in liquid media, the colonies were observed morphologically on the solid media. Smears of both strains were observed under microscope after Giemsa stain.
Results :
In the liquid media, glucose concentrations had effect on both clinical corneal isolated and standard A. fumigatus stains (P<0.01), while clinical isolated strain grew better under glucose pressure. Without peptone, both strains grew slowly and had no significant difference (P=0.064). On the solid media, the colonies' morphology of clinical isolated A. fumigatus under low glucose concentrations (0.00%-0.15%) were similar, while the colonies' morphology of standard strain was affected by glucose concentration significantly. Without peptone, the clinical isolated colonies grew better than the standard one.
Conclusions :
Under different glucose and nitrogen pressure, clinical corneal isolated A. fumigatus has stronger environmental adaptive capacity, invasive ability, and microbial dispersion ability.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.