July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Protective mechanisms of polyphenolic-enriched extract of plants against H2O2-induced oxidative stress in human retinal pigment epithelial cells
Author Affiliations & Notes
  • Hachemi Nezzar
    Ophthalmology Dept., Dubai Health Authority, DH, Dubai, United Arab Emirates
    neuroscience, IGCNC-728, Image Guided Clinical Neuroscience and Connectomics,, Clermont-Ferrand France, France
  • stephanie Hermitte
    CNRS, LMGE, Université Clermont Auvergne, CNRS, LMGE, CLERMONT FERRAND, France
  • pascale goupil
    UMR 547-UBP/INRA PIAF, CLERMONT FERRAND, France, France
  • Claire Richard
    CNRS, SIGMA Clermont, ICCF, CLERMONT FERRAND, France
  • Mohamad Sleiman
    CNRS, SIGMA Clermont, ICCF, CLERMONT FERRAND, France
  • Elodie Peghaire
    CNRS, LMGE, Université Clermont Auvergne, CNRS, LMGE, CLERMONT FERRAND, France
  • Hicham El Alaoui
    CNRS, LMGE, Université Clermont Auvergne, CNRS, LMGE, CLERMONT FERRAND, France
  • Niyazi Acar
    Centre des Sciences du Goût et de l'Alimentation,Nutrition and eye research group, INRA, Dijon, France
  • ayhan kocer
    Laboratoire GReD "Génétique, Reproduction & Développement", UMR CNRS 6293, INSERM U1103, CLERMONT FERRAND, France
  • Footnotes
    Commercial Relationships   Hachemi Nezzar, None; stephanie Hermitte, None; pascale goupil, None; Claire Richard, None; Mohamad Sleiman, None; Elodie Peghaire, None; Hicham El Alaoui, None; Niyazi Acar, None; ayhan kocer, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5377. doi:
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      Hachemi Nezzar, stephanie Hermitte, pascale goupil, Claire Richard, Mohamad Sleiman, Elodie Peghaire, Hicham El Alaoui, Niyazi Acar, ayhan kocer; Protective mechanisms of polyphenolic-enriched extract of plants against H2O2-induced oxidative stress in human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5377.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The current study focused on the protective effect of polyphenolic compounds derived from plants extracts against oxidative stress on human retinal pigment epithelial cells (ARPE19) in order to mimic age-related macular degeneration (AMD)

Methods : ARPE19 cells were cultured in the presence of various concentrations of polyphenolic compounds (PP1-PP12) derived from plants extracts. ARPE19 cells were pretreated for 24 hours before being exposed to hydrogen peroxide (H2O2) for 2 hours to induce oxidative stress. Cell metabolic activity was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The lipid peroxidation was evaluated using TBARS assays. The expression of the oxidative stress-related gene was determined by real-time reverse transcription polymerase chain reaction

Results : Pretreatment of ARPE19 cells with polyphenolic compounds (PP1-PP12) alone does not affect the cell viability up to 100µM. Exposure of ARPE19 cells to 300 µM of H2O2 caused a significant decrease in cell viability, whereas pretreatment with 5 and 50 µM significantly prevented this decrease. Moreover, the viability of pretreated cells with certain compounds (PP1 and PP4) seems to be significantly increased in cultures treated with H2O2 alone. In addition, pretreated cells displayed significantly decreased of lipid peroxidation products compared to the control. The oxidative stress related expressions are decreased in pretreated cell with the polyphenolic compounds

Conclusions : Our results show that the pretreatment with selected polyphenolic compounds can protect ARPE19 cells against H2O2-induced oxidative damage and death. These results suggest a potential of these natural compounds in the prevention of retinal diseases associated with oxidative stress. Further studies should be encouraged to explore the potential use of polyphenolic compounds as a suitable strategy for the prevention or treatment of AMD

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

 

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