July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
The effect of mobile zinc on retinal ganglion cells death after glaucomatous optic nerve injury
Author Affiliations & Notes
  • Zhe Liu
    Zhongshan Ophthalmic Center, SYSU, Guangzhou, Guangdong, China
  • Li Yangjiani
    Zhongshan Ophthalmic Center, SYSU, Guangzhou, Guangdong, China
  • Yunan Wei
    Zhongshan Ophthalmic Center, SYSU, Guangzhou, Guangdong, China
  • Jingfei Xue
    Zhongshan Ophthalmic Center, SYSU, Guangzhou, Guangdong, China
  • Yehong Zhuo
    Zhongshan Ophthalmic Center, SYSU, Guangzhou, Guangdong, China
  • Yiqing Li
    Zhongshan Ophthalmic Center, SYSU, Guangzhou, Guangdong, China
  • Footnotes
    Commercial Relationships   Zhe Liu, None; Li Yangjiani, None; Yunan Wei, None; Jingfei Xue, None; Yehong Zhuo, None; Yiqing Li, None
  • Footnotes
    Support  National Natural Science Foundation of China (81870657), Natural Science Foundation of Guangdong province (2018A030313049)
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 630. doi:
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      Zhe Liu, Li Yangjiani, Yunan Wei, Jingfei Xue, Yehong Zhuo, Yiqing Li; The effect of mobile zinc on retinal ganglion cells death after glaucomatous optic nerve injury. Invest. Ophthalmol. Vis. Sci. 2019;60(9):630.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Traditional therapies targeting RGCs failed to maintain the long-term cell survival or axon regeneration in glaucoma, which suggested unknown molecular mechanisms exist. Our previous research revealed that mobile zinc (Zn2+) increased rapidly in the early stage after injury directly to the optic nerve and mediated RGCs apoptosis (Li Y, et al. PNAS 2017). In addition, Zn2+ chelation enabled long survival of RGCs and axon regeneration. However, the effect of Zn2+ on RGCs death in the glaucoma model has not been fully elucidated.

Methods : 1. A mouse model of acute ocular hypertension (OH, 80 mmHg for 1 hour) was established, and the level and localization of Zn2+ in retina were monitored by autometallography (AMG) 6 hours post injury.
2. RGC-5 was cultured and treated with Zn2+in vitro. We studied the time-dependent effect of Zn2+ on RGCs apoptosis at 6h, 12h and 18h using flow cytometry labeled by Annexin V-FITC/PI.
3. The dose-dependent effect of Zn2+ (50, 100, 150 μM) on mitochondrial function was revealed by detecting fluorescenct level of JC-1, a fluorescence probe which reflects mitochondrial membrane potential with different color.
4. The oxygen-glucose deprivation for 3h and reperfusion for 6h (OGD/R model) was established on primary cultured mouse RGCs, and the effect of specific Zn2+ chelator ZX1 on RGCs apoptosis was detected by flow cytometry.

Results : 1. After OH, the level of Zn2+ increased rapidly (1.76±0.26-fold, P<0.05) in the inner-plexiform layer within 6 hours (Fig. 1).
2. 6 hours after Zn2+ treatment (150 μM) in vitro, significant death of RGC-5 was observed (15.32 ±0.32 %, P<0.05). The toxicity of Zn2+ increased in a time-dependent manner (Fig. 2A).
3. The mitochondrial membrane potential declined in accordance with Zn2+ concentration, and the toxicity of Zn2+ increased in a dose-dependent manner (Fig. 2B)
4. Zn2+ Chelator ZX1 (5 μM) significantly inhibited the apoptosis of RGCs in OGD/R model (P<0.0001, Fig. 2C).

Conclusions : Mobile Zn2+ increased rapidly in mouse retina after OH, leading to RGCs injury. Zn2+ chelators significantly enhanced the survival of RGCs. Apoptosis mediated by mitochondrial disfunction could be the mechanism underlying Zn2+ toxicity. This study provides a new and promising therapeutic target for glaucoma.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

Zn2+-AMG staining in mouse retina at 6h after ocular hypertension.

Zn2+-AMG staining in mouse retina at 6h after ocular hypertension.

 

Zn2+ induced cell death through mitochondrial disfunction in cultured retinal neurons.

Zn2+ induced cell death through mitochondrial disfunction in cultured retinal neurons.

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