July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Quantifying spherical aberration of the human crystalline lens during simulated accommodation using ray-tracing aberrometry
Author Affiliations & Notes
  • Marco Ruggeri
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ of Miami, Miami, Florida, United States
  • Siobhan Williams
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ of Miami, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Bianca Maceo Heilman
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ of Miami, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Ashik Mohamed
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, India
    School of Optometry and Vision Science, The University of New South Wales, Sydney, New South Wales, Australia
  • Yu-Cherng Chang
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ of Miami, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • N. Geetha Sravani
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, India
  • Shravya Sri Durgam
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, India
  • Arthur Ho
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ of Miami, Miami, Florida, United States
    Brien Holden Vision Institute, Sydney, New South Wales, Australia
  • Jean-Marie Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ of Miami, Miami, Florida, United States
    Vision Cooperative Research Centre, Sydney, New South Wales, Australia
  • Fabrice Manns
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ of Miami, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Footnotes
    Commercial Relationships   Marco Ruggeri, None; Siobhan Williams, None; Bianca Maceo Heilman, None; Ashik Mohamed, None; Yu-Cherng Chang, None; N. Geetha Sravani, None; Shravya Durgam, None; Arthur Ho, None; Jean-Marie Parel, None; Fabrice Manns, None
  • Footnotes
    Support  National Eye Institute (R01EY021834; 1F30-EY027162; F31EY021444); Ruth L. Kirschstein National Research Service Award Individual Pre-doctoral Fellowship; BMH; P30EY14801 (Center Core Grant); the Florida Lions Eye Bank and Beauty of Sight Foundation; the Henri and Flore Lesieur Foundation (JMP); Drs. Raksha Urs and Aaron Furtado; Karl R. Olsen, MD and Martha E. Hildebrandt, PhD; an unrestricted grant from Research to Prevent Blindness; Hyderabad Eye Research Foundation; Australian Federal Government Cooperative Research Centre Scheme through the Vision Cooperative Research Centre.
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3170. doi:
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      Marco Ruggeri, Siobhan Williams, Bianca Maceo Heilman, Ashik Mohamed, Yu-Cherng Chang, N. Geetha Sravani, Shravya Sri Durgam, Arthur Ho, Jean-Marie Parel, Fabrice Manns; Quantifying spherical aberration of the human crystalline lens during simulated accommodation using ray-tracing aberrometry. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3170.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To demonstrate the viability of using a ray-tracing aberrometer to measure spherical aberration of the human crystalline lens in vitro during simulated accommodation.

Methods : A custom-made system that merges ray-tracing aberrometry and OCT (Ruggeri et al, Biomed Opt Express 2018;9:3834-51) was used to measure the optical aberrations and geometry of the human lens. Experiments were performed on 5 post-mortem lenses (Ages: 32, 34, 34, 45 and 45 y/o). The lens with its accommodative framework was mounted in a stretcher and submerged in a chamber filled with BSS. Simulated accommodation was achieved by radially stretching the globe in 2 mm increments. The aberrometer delivered a matrix of 13 × 13 rays spaced 0.5 mm apart through the lens for each stretching state. A 2D image sensor recorded images of the spots generated by the exit rays. Images of the spot patterns acquired at 9 different axial positions were processed to calculate the slope of the exit rays. Ray slopes over a 6 mm pupil were used to calculate Zernike wavefront coefficients. Lens power and spherical aberration (SA) were determined from the defocus and 4th order Zernike terms, respectively. Lens thickness was calculated from the OCT images. The system enables measurements with a precision of 0.4 D (power), 0.06 µm (SA) and 10 µm (thickness).

Results : Zernike coefficients (defocus and SA), power and thickness are summarized in the table for each lens and stretching state. Lens power and thickness increase with simulated accommodation (decreased stretch) in the younger lenses (32 and 34 y/o), as expected. In the older lenses (45 y/o), there were no measured changes in shape and power with stretching. There were no measured changes in spherical aberration with stretching across lenses. Spherical aberration was significantly less negative in the older lenses (45 y/o), which is consistent with prior results (Smith et al, Vision Res 2001;41:235-43).

Conclusions : We assessed the feasibility of measuring spherical aberration of the human lens in vitro during simulated accommodation with a ray-tracing aberrometer. Simulated accommodation had no effect on spherical aberration in lenses older than ~30 y/o.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

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