July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Changes in Staphylococcus aureus Virulence Factors and Host Immune Response Following Rose Bengal Photodynamic Antimicrobial Therapy
Author Affiliations & Notes
  • Heather Durkee
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Alejandro Arboleda
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Jorge Maestre-Mesa
    Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Mariela Aguilar
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Guillermo Amescua
    Anne Bates Leach Eye Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Jean-Marie Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Darlene Miller
    Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Footnotes
    Commercial Relationships   Heather Durkee, None; Alejandro Arboleda, None; Jorge Maestre-Mesa, None; Mariela Aguilar, None; Guillermo Amescua, None; Jean-Marie Parel, None; Darlene Miller, None
  • Footnotes
    Support  University of Miami Scientific Awards Committee Interdisciplinary Team Science Pilot Award (UM SAC 2016-17), Florida Lions Eye Bank and Beauty of Sight Foundation, Edward D and Janet K Robson Fund, NIH Center Grant P30EY14801, Research to Prevent Blindness, Henri and Flore Lesieur Foundation (JMP), Drs Raksha Urs and Aaron Furtado, and The Brien Holden Vision Institute.
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 838. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Heather Durkee, Alejandro Arboleda, Jorge Maestre-Mesa, Mariela Aguilar, Guillermo Amescua, Jean-Marie Parel, Darlene Miller; Changes in Staphylococcus aureus Virulence Factors and Host Immune Response Following Rose Bengal Photodynamic Antimicrobial Therapy. Invest. Ophthalmol. Vis. Sci. 2019;60(9):838.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Staphylococcus aureus (S. aureus) virulence factors as well as the host inflammatory response makes management challenging. Optimal therapy would neutralize the S. aureus toxins while reducing the host immune response. One such therapy under investigation is Photodynamic Antimicrobial Therapy (PDAT). The impact of PDAT on the S. aureus virulence factors and the host immune response remains unknown. The purpose of this study is to measure the changes in: (1) virulence factors of two S. aureus isolates and (2) the subsequent immune response in an ex-vivo EpiCorneal tissue model following rose bengal PDAT.

Methods : (1) Virulence Factors: One methicillin-resistant S. aureus (MRSA) and one methicillin-susceptible S. aureus (MSSA) isolate were collected from patients with infectious keratitis. The inocula (105 CFU/mL) were mixed with either water or 0.1% rose bengal, and plated in triplicate onto blood agar. Plates with rose bengal were irradiated with a custom-made green LED light source. Organisms were collected from each plate, DNA was extracted, and virulence factors were quantified by metagenomics (CosmosID). (2) Host Immune Response: Evaluated using EpiCorneal tissue model (MatTek). Control, Infection, and Infection-PDAT groups were tested in duplicate. Infection and Infection-PDAT groups were inoculated using the same S. aureus isolates described above. In the Infection-PDAT group, 0.1% rose bengal was applied and irradiated as described above. Fluids from the groups were collected and IL-1β, -6 and -8 quantified with ELISA.

Results : The frequency of the 35 virulence factors varied among the MRSA (12/35) and MSSA (21/35) strains. The subgroup of virulence factors relating to leukocyte migration and phagocytosis (SSL-5, -7,-10,-11, CHIPS, SOX) were more expressed in MSSA compared to MRSA, except SSL-11, and all were downregulated following rose bengal PDAT. Furthermore, the host immune response was higher in the S. aureus Infection groups compared to Control group. IL-8 showed the greatest response, followed by IL-6 and IL-1β. In the Infection-PDAT group, the largest downregulation of immune factors was observed in the MSSA strain.

Conclusions : This study demonstrates that virulence factors of S. aureus isolates and the subsequent immune response in EpiCorneal tissue models vary among S. aureus strains and are altered following rose bengal PDAT.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×