July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Normative retrobulbar measurements of the optic nerve using ultra high field magnetic resonance imaging
Author Affiliations & Notes
  • Bao N Nguyen
    Department of Optometry and Vision Sciences, The University of Melbourne, University of Melbourne, Victoria, Australia
  • Jon O Cleary
    Melbourne Brain Centre Imaging Unit, Department of Anatomy and Neuroscience, The University of Melbourne, Victoria, Australia
    Department of Radiology, Guy’s and St. Thomas’ NHS Foundation Trust, London, United Kingdom
  • Rebecca Glarin
    Melbourne Brain Centre Imaging Unit, Department of Anatomy and Neuroscience, The University of Melbourne, Victoria, Australia
    Department of Radiology, Royal Melbourne Hospital, Victoria, Australia
  • Scott C Kolbe
    Melbourne Brain Centre Imaging Unit, Department of Anatomy and Neuroscience, The University of Melbourne, Victoria, Australia
  • Bradford A Moffat
    Melbourne Brain Centre Imaging Unit, Department of Anatomy and Neuroscience, The University of Melbourne, Victoria, Australia
  • Roger J Ordidge
    Melbourne Brain Centre Imaging Unit, Department of Anatomy and Neuroscience, The University of Melbourne, Victoria, Australia
  • Bang V Bui
    Department of Optometry and Vision Sciences, The University of Melbourne, University of Melbourne, Victoria, Australia
  • Allison M McKendrick
    Department of Optometry and Vision Sciences, The University of Melbourne, University of Melbourne, Victoria, Australia
  • Footnotes
    Commercial Relationships   Bao Nguyen, None; Jon Cleary, None; Rebecca Glarin, None; Scott Kolbe, None; Bradford Moffat, None; Roger Ordidge, None; Bang Bui, None; Allison McKendrick, None
  • Footnotes
    Support  Melbourne Neuroscience Institute Fellowship (BNN), University of Melbourne Mackenzie Fellowship (JOC), Melbourne Neuroscience Institute Interdisciplinary Seed Funding Grant (AMM, BVB, RJO). The 7T system at MBCIU is supported by the Australian National Imaging Facility​.
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 6109. doi:
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    • Get Citation

      Bao N Nguyen, Jon O Cleary, Rebecca Glarin, Scott C Kolbe, Bradford A Moffat, Roger J Ordidge, Bang V Bui, Allison M McKendrick; Normative retrobulbar measurements of the optic nerve using ultra high field magnetic resonance imaging. Invest. Ophthalmol. Vis. Sci. 2019;60(9):6109.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We exploit the improved spatial resolution and signal-to-noise gain of ultra high field (7T) magnetic resonance imaging (MRI) with a dedicated eye coil for more accurate morphometric measurements of the optic nerve ~2.5mm behind the globe.

Methods : Coronal T2-weighted oblique images (TR=2000ms, TE=64ms, FOV=155mm, matrix=384 x 384, slice thickness=0.7mm, scan time=2’34”) through the optic nerve were obtained in 21 healthy adults (20-41 years, 11 emmetropes: +0.75 to -0.50D, 10 myopes: -4.5 to -12D) using a 7T Siemens Magnetom scanner (Erlangen, Germany) and 6-channel eye coil (MRI.TOOLS GmbH, Berlin, Germany). Horizontal and vertical outer diameter of the optic nerve, subarachnoid space (fluid gap) and optic sheath were measured by hand using biomedical imaging software (OsiriX, Pixmeo, Switzerland) (Figure). Significant motion artefacts were avoided with customised fixation and preparation techniques.

Results : Horizontal and vertical measurements were similar so were averaged. Right and left eye diameters did not differ and were highly correlated (optic nerve: Pearson r=0.9, p<0.001; fluid gap: r=0.8, p<0.001; optic sheath: r=0.7, p<0.001); hence we report left eye data only. Optic nerve diameter (average of horizontal and vertical diameters) ranged from 2.8-4.1mm in emmetropes and 1.5-4.2mm in myopes and correlated with refractive error (Spearman r=0.46, p=0.04). Similarly, fluid gap diameter (emmetropes: 3.6-5.5mm, myopes: 2.5-5.6mm), but not optic sheath diameter (emmetropes: 4.5-6.8mm, myopes: 4.2-6.8mm), correlated with refractive error (r=0.47, p=0.03).

Conclusions : Ultra high field MRI with thinner slices enables more accurate demarcation of the optic nerve, surrounding fluid/subarachnoid space and optic sheath without overlapping of neighbouring anatomy (minimal partial volume artefact). Our 7T MRI-derived normative measurements of optic nerve, fluid gap and sheath diameter are comparable with published reports in healthy observers obtained at conventional MRI magnetic fields (1.5-3T). Our findings suggest a trend for retrobulbar optic nerve and subarachnoid space, but not optic sheath, to be smaller in high myopes.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

Example left eye coronal oblique scan through the optic nerve indicating the three anatomical areas of interest (1) optic nerve (2) fluid gap (3) optic sheath

Example left eye coronal oblique scan through the optic nerve indicating the three anatomical areas of interest (1) optic nerve (2) fluid gap (3) optic sheath

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