July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Removal of suture-induced corneal neovascularization in rabbits using concurrently applied nanosecond laser pulses and ultrasound
Author Affiliations & Notes
  • Yixin Yu
    Department of Ophthalmology and Visual science, University of Michigan, Ann Arbor, Michigan, United States
    Department of Ophthalmology, Xiangya Hospital, Changsha, Hunan, China
  • Xinyi Xie
    Department of Ophthalmology and Visual science, University of Michigan, Ann Arbor, Michigan, United States
  • Yu Qin
    Department of Ophthalmology and Visual science, University of Michigan, Ann Arbor, Michigan, United States
  • Xiaobo Xia
    Department of Ophthalmology, Xiangya Hospital, Changsha, Hunan, China
  • Maria A Woodward
    Department of Ophthalmology and Visual science, University of Michigan, Ann Arbor, Michigan, United States
  • Xinmai Yang
    Mechanical Engineering, University of Kansas, Kansas, Kansas, United States
  • Xueding Wang
    Biomedical Engineering, University of Michigan, Michigan, United States
  • Yannis Mantas Paulus
    Department of Ophthalmology and Visual science, University of Michigan, Ann Arbor, Michigan, United States
    Biomedical Engineering, University of Michigan, Michigan, United States
  • Footnotes
    Commercial Relationships   Yixin Yu, None; Xinyi Xie, None; Yu Qin, None; Xiaobo Xia, None; Maria Woodward, None; Xinmai Yang, None; Xueding Wang, None; Yannis Paulus, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 938. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yixin Yu, Xinyi Xie, Yu Qin, Xiaobo Xia, Maria A Woodward, Xinmai Yang, Xueding Wang, Yannis Mantas Paulus; Removal of suture-induced corneal neovascularization in rabbits using concurrently applied nanosecond laser pulses and ultrasound. Invest. Ophthalmol. Vis. Sci. 2019;60(9):938.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Pathologic corneal neovascularization (CNV) is potential consequence of various disorders such as dry eye, infection, trauma and surgery. This report describes a novel therapy to selectively treat CNV using a combination of low intensity nanosecond pulse duration laser and ultrasound termed photo-mediated ultrasound therapy (PUT).

Methods : An integrated therapeutic ultrasound, laser, and CCD camera system was devised. A Nd:YAG laser produced laser pulses with 5-ns pulse duration and 10-Hz pulse repetition rate at 532 nm at the beginning of each ultrasound burst focused on the cornea with 5mm spot size. Twelve New Zealand white rabbits were treated with one 7-0 nylon suture surgically placed partial thickness in the cornea to generate a rabbit CNV model. Six rabbits were treated with multiple PUT settings and 6 served as controls. Rabbits were monitored for up to 3 weeks after PUT treatment with photography and fluorescein angiography (FA).

Results : The control group resulted in no appreciable vascular changes with persistent CNV (Fig 1). PUT resulted in the immediate removal of CNV in the treatment area. CNV returned gradually after PUT treatment but remained decreased in the region of treatment up to 1 week following PUT. By 3 weeks, CNV had returned to baseline before PUT treatment. FA demonstrates that the region of treatment had the immediate removal of blood vessel perfusion after PUT treatment that remained diminished at 1 week and returned to normal by 3 weeks (Fig 2).

Conclusions : With the capability to precisely treat a large area of corneal neovascularization PUT holds promise as a novel, non-invasive method to treat corneal neovascularization.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

Figure 1. Vessel-removal effects of non-treatment on rabbit cornea neovascularization. Red-free of the cornea before treatment (a), FA of the cornea before treatment (d). Photos after treatment by 7 day (b,e) and 21 days (c,f).

Figure 1. Vessel-removal effects of non-treatment on rabbit cornea neovascularization. Red-free of the cornea before treatment (a), FA of the cornea before treatment (d). Photos after treatment by 7 day (b,e) and 21 days (c,f).

 

Figure 3. Vessel-removal effects of PUT treatment on rabbit cornea neovascularization. FA of the cornea before treatment (a) and after treatment showing a white nonperfused region immediately (b) and 7 days (c). FA of the cornea before treatment (e), after treatment showing a white nonperfused region immediately (b,f) and 7days (c,g) and after treatment showing CNV return to normal by 21 days (d,h). (Red circle indicates treatment area)

Figure 3. Vessel-removal effects of PUT treatment on rabbit cornea neovascularization. FA of the cornea before treatment (a) and after treatment showing a white nonperfused region immediately (b) and 7 days (c). FA of the cornea before treatment (e), after treatment showing a white nonperfused region immediately (b,f) and 7days (c,g) and after treatment showing CNV return to normal by 21 days (d,h). (Red circle indicates treatment area)

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×