July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
In vivo visualization of inner plexiform layer lamination by visible light OCT with spectral shaping
Author Affiliations & Notes
  • Tingwei Zhang
    Biomedical Engineering Department, University of California, Davis, Davis, California, United States
  • Jose Garcia
    Electrical and Computer Engineering Department, University of California, Davis, Davis, California, United States
  • Vivek Jay Srinivasan
    Biomedical Engineering Department, University of California, Davis, Davis, California, United States
    Ophthalmology and Vision Science, University of California, Davis, Sacramento, California, United States
  • Footnotes
    Commercial Relationships   Tingwei Zhang, None; Jose Garcia, None; Vivek Srinivasan, Optovue (P)
  • Footnotes
    Support  R01EY028287; Glaucoma Research Foundation Catalyst for a Cure
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1303. doi:
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    • Get Citation

      Tingwei Zhang, Jose Garcia, Vivek Jay Srinivasan; In vivo visualization of inner plexiform layer lamination by visible light OCT with spectral shaping. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1303.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Retinal ganglion cell (RGC) loss is a hallmark of glaucoma, and RGC dendrites which stratify in the off sublamina of the inner plexiform layer (IPL) may exhibit early glaucomatous changes. However, the in vivo visualization and quantification of the IPL remains challenging due to insufficient achievable contrast. Here, we used our achromatized visible light OCT, with a hardware spectral shaping method, to reduce sidelobes in the point spread function (PSF). We tested whether this technology can visualize the subtle differences in reflectivity due to IPL lamination.

Methods : A fiber-based, spectral/Fourier domain visible light OCT system for human retinal imaging was built. To reduce PSF sidelobes, we developed a diffractive optical setup to shape the spectrum (Figure 1A). The broadband light is spatially dispersed and focused on a grating light valve spatial light modulator (GLV-SLM). With appropriate control of the GLV-SLM, the original spectrum can be shaped to a desired spectrum. The GLV-SLM can also reduce the short wavelength light exposure during subject alignment. A scanning protocol with continuous volumes spaced along the final image axis was used. Then, two dimensional motion correction and averaging were applied to generate one cross-sectional image.

Results : In Figure 1B, the original spectrum was shaped to Hamming window with 565 nm center wavelength for ultrahigh resolution (UHR) imaging, and 625 nm center wavelength for alignment. The sidelobes of the axial PSF are suppressed (Figure 1C). Our preliminary result (Figure 2) indicates that human IPL lamination can be resolved.

Conclusions : We developed a novel spectral shaping method to further increase the contrast of visible light OCT. IPL lamination was clearly resolved. This method may provide additional morphological information to diagnose and monitor retinal diseases and glaucoma.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

(A) Spectral shaping optical setup. (B) Original and shaped spectra, for both UHR imaging and subject alignment. (C) PSFs for both the original spectrum and the shaped, UHR spectrum.

(A) Spectral shaping optical setup. (B) Original and shaped spectra, for both UHR imaging and subject alignment. (C) PSFs for both the original spectrum and the shaped, UHR spectrum.

 

(A) Ultrahigh resolution visible light OCT image with stretched contrast. A hyporeflective band is resolved (red arrow), which appears clearer in the flattened image (B). Another hyporeflective band is occasionally resolved (green arrow). The averaged, linear signal amplitude profile (C) and individual signal amplitude profiles (D) of the IPL are shown.

(A) Ultrahigh resolution visible light OCT image with stretched contrast. A hyporeflective band is resolved (red arrow), which appears clearer in the flattened image (B). Another hyporeflective band is occasionally resolved (green arrow). The averaged, linear signal amplitude profile (C) and individual signal amplitude profiles (D) of the IPL are shown.

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