July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Quantitative Fundus Autofluorescence (qAF) levels of two subtypes of geographic atrophy (GA) secondary to age-related macular degeneration (AMD)
Author Affiliations & Notes
  • R Theodore Smith
    Ophthalmology, Icahn School of Medicine of Mount Sinai, New York, New York, United States
  • Marco Mazzola
    New York Eye and Ear Infirmary of Mount Sinai, New York, United States
    Department of Medicine and Surgery, University of Insubria Varese-Como, Italy
  • Diane Wang
    New York University School of Medicine, New York, United States
  • Wei Wei
    New York Eye and Ear Infirmary of Mount Sinai, New York, United States
  • K Bailey Freund
    Ophthalmology, Vitreous Retina Macula Consultants, New York, United States
  • Footnotes
    Commercial Relationships   R Theodore Smith, None; Marco Mazzola, None; Diane Wang, None; Wei Wei, None; K Bailey Freund, None
  • Footnotes
    Support  NIH R01 EY015520
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3470. doi:
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      R Theodore Smith, Marco Mazzola, Diane Wang, Wei Wei, K Bailey Freund; Quantitative Fundus Autofluorescence (qAF) levels of two subtypes of geographic atrophy (GA) secondary to age-related macular degeneration (AMD). Invest. Ophthalmol. Vis. Sci. 2019;60(9):3470.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : GA, the advanced dry form of AMD, progresses relentlessly but with remarkable interindividual variation. AF is the preferred method to evaluate GA lesion size and growth. qAF further calibrates the AF image intensity to an embedded fluorescence reference, to quantify and compare AF levels between patients and across time (Smith, IOVS, 2011). We sought to divide GA by qAF levels and predominant AMD lesion type into two clinically useful phenotypes.

Methods : 25 Regions-of-interest (ROIs) of 9 eyes of 8 pseudophakic patients with GA secondary to non-neovascular AMD were studied. ROIs included all atrophic lobules in each eye.
We performed near-infrared (NIR), spectral-domain optical coherence tomography (SD-OCT) and qAF imaging. Eyes and ROIs were divided into 2 groups according to the predominant intermediate AMD (iAMD) lesion (soft drusen or subretinal drusenoid deposits (SDD)) on SD-OCT. Mean qAF was then computed for the two groups of ROI.

Results : Phenotype 1, soft drusen and GA. GA lesions are isolated and “black” on AF, with lower qAF (mean qAF 22.175, SD3.46)
Phenotype 2, SDD and GA. GA lesions are multifocal and “gray” on AF, with higher qAF (mean qAF 54.63, SD 16.74, p=0.0006)

Conclusions : GA may be conveniently and simply divided by the predominant associated iAMD lesion (soft drusen or SDD) and the qAF levels of the GA lobules into 2 main, non-exclusive phenotypes that may help define two pathways of GA pathogenesis, allow more refined phenotype-genotype correlation, and enable an individualized patient prognosis.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

GA in an SDD phenotype (83-year-old woman). Lobules of GA, yellow, in the qAF image. Yellow lines indicate location of SD-OCT B-scan. Purple boxes on near infrared (NIR) image and corresponding SD-OCT, with myriad SDD.

GA in an SDD phenotype (83-year-old woman). Lobules of GA, yellow, in the qAF image. Yellow lines indicate location of SD-OCT B-scan. Purple boxes on near infrared (NIR) image and corresponding SD-OCT, with myriad SDD.

 

GA in a soft drusen phenotype (86-year-old woman). One lobule of GA, yellow, in the qAF image. Yellow lines indicate location of SD-OCT B-scan. Purple boxes on NIR image and corresponding SD-OCT, with soft drusen.

GA in a soft drusen phenotype (86-year-old woman). One lobule of GA, yellow, in the qAF image. Yellow lines indicate location of SD-OCT B-scan. Purple boxes on NIR image and corresponding SD-OCT, with soft drusen.

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