July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Scleral crosslinking using genipin can effect normal eye development in tree shrews
Author Affiliations & Notes
  • Mustapha El Hamdaoui
    The University of Alabama at Birmingham, Birmingham, Alabama, United States
  • Mokshad Gaonkar
    The University of Alabama at Birmingham, Birmingham, Alabama, United States
  • Christopher A Girkin
    The University of Alabama at Birmingham, Birmingham, Alabama, United States
  • Brian C Samuels
    The University of Alabama at Birmingham, Birmingham, Alabama, United States
  • Rafael Grytz
    The University of Alabama at Birmingham, Birmingham, Alabama, United States
  • Footnotes
    Commercial Relationships   Mustapha El Hamdaoui, None; Mokshad Gaonkar, None; Christopher Girkin, None; Brian Samuels, None; Rafael Grytz, None
  • Footnotes
    Support  NIH Grant R01-EY026588; EyeSight Foundation of Alabama; Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5862. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Mustapha El Hamdaoui, Mokshad Gaonkar, Christopher A Girkin, Brian C Samuels, Rafael Grytz; Scleral crosslinking using genipin can effect normal eye development in tree shrews. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5862. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : This study is aimed at investigating the effect of scleral crosslinking (SXL) using sub-Tenon’s injections of genipin in tree shrews eyes with normal visual experience.

Methods : Five injections of 0.4 mL of genipin at 0 mM (sham) and 20 mM were performed in one eye while the fellow eye was considered as control. Injections were carried out over 15 min every other day starting at 18 days of visual experience (DVE). Tree shrews were randomly assigned into 3 experimental groups: normal visual experience without any injections (n=6), with 5 x sham injections (n=6) and with 5 x genipin injections at 20 mM (n=6). Daily measurements of axial dimensions and the refractive state were performed in awake animals between 18 DVE and 35 DVE. A mixed-design repeated measures ANOVA model was used to test for mean differences in axial dimensions and refractive state between the different groups.

Results : Compared to the normal group, the sham injection group showed a significant reduction in vitreous chamber depth and axial length (p<0.05). Compared to the sham group, genipin injected eyes showed significant corneal thickening and a significant reduction in anterior chamber depth, vitreous chamber depth and axial length (p<0.05). Surprisingly, the refractive state was not significantly different between any of the tested groups.

Conclusions : We have shown that scleral crosslinking using genipin can alter the development of axial dimensions in a normal tree shrew eyes. We have shown that sub-Tenon’s sham injections reduce the vitreous chamber depth. This effect is increased when genipin is used. In contrast to sham injections, SXL also impacted the development of the anterior chamber depth and corneal thickness. The observed corneal swelling in the genipin group might have caused changes in corneal curvature, which counteracted the reduction in axial length and kept the refractive state unchanged. Further optimization of the genipin delivery approach is needed to reduce the effect of scleral crosslinking on the anterior segment of the eye.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

Development of the refractive error (A) and changes in axial dimensions (B-F) in the six experimental groups. Plotted are the differences between treated and control eyes (right-left for the normal group). Arrows mark the days where sub-Tenon’s injections were performed. * indicates a significant difference between groups (p < 0.05).

Development of the refractive error (A) and changes in axial dimensions (B-F) in the six experimental groups. Plotted are the differences between treated and control eyes (right-left for the normal group). Arrows mark the days where sub-Tenon’s injections were performed. * indicates a significant difference between groups (p < 0.05).

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×