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xuan liu, Martin Zinkernagel, Petra Schwarzer, Despina Kokona; Multimodal imaging of experimental choroidal neovascularization. Invest. Ophthalmol. Vis. Sci. 2019;60(11):PB0146. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To compare different imaging modalities for assessing experimental choroidal neovascularization in rodents.
Experimental choroidal neovascularization (eCNV) was induced by rupturing Bruch’s membrane with a laser. Multimodal imaging, including fluorescein angiography (FA), cSLO multicolor imaging (including blue reflectance (BR; 488 nm), green reflectance (GR; 515 nm) and infrared reflectance (IR; 820 nm) ) as well as optical coherence tomography angiography were used to quantify the presumed area of eCNV 7 and 14 days after laser. At day 14, multimodal imaging was compared with histopathologic measurements from isolectin-stained choroidal flatmounts.
Choroidal neovascularization area measurements showed a decrease in all imaging modalities between day 7 and 14. Correlation between different imaging modalities at day 7 showed that FA and multicolor imaging correlated best (r=0.4, p<0.05). At day 14, there was a high correlation between area measurements in flatmounts and FA (r=0.6, p<0.001) and multicolor imaging (r=0.4, p<0.05). Area measurements of OCTA with other imaging modalities was poor both at day 7 and 14.
FA quantification of CNV area correlated best with CNV area measured in isolectin stained choroidal flatmounts and remains the gold standard to quantify CNV area in vivo.
This abstract was presented at the 2019 ARVO Imaging in the Eye Conference, held in Vancouver, Canada, April 26-27, 2019.
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