August 2019
Volume 60, Issue 11
Free
ARVO Imaging in the Eye Conference Abstract  |   August 2019
Morphological changes in Bruch’s membrane opening during intraocular pressure elevation
Author Affiliations & Notes
  • Yanhui Ma
    Department of Biomedical Engineering, The Ohio State University , Columbus, Ohio, United States
  • Sunny Kwok
    Department of Biomedical Engineering, The Ohio State University , Columbus, Ohio, United States
  • Keyton Clayson
    Department of Biomedical Engineering, The Ohio State University , Columbus, Ohio, United States
    Biophysics Interdisciplinary Group, The Ohio State University, Columbus, Ohio, United States
  • Xueliang Pan
    Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, United States
  • Jun Liu
    Department of Biomedical Engineering, The Ohio State University , Columbus, Ohio, United States
    Department of Ophthalmology and Visual Science, The Ohio State University, Columbus, Ohio, United States
  • Footnotes
    Commercial Relationships   Yanhui Ma, None; Sunny Kwok, None; Keyton Clayson, None; Xueliang Pan, None; Jun Liu, None
  • Footnotes
    Support  RO1EY020929 and RO1EY025358
Investigative Ophthalmology & Visual Science August 2019, Vol.60, PB0181. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yanhui Ma, Sunny Kwok, Keyton Clayson, Xueliang Pan, Jun Liu; Morphological changes in Bruch’s membrane opening during intraocular pressure elevation. Invest. Ophthalmol. Vis. Sci. 2019;60(11):PB0181.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To quantify the changes in the location and diameter of Bruch’s membrane opening (BMO) in response to acute intraocular pressure (IOP) elevation by using ultrasound imaging.

Methods : Whole globe inflation tests were performed in 7 human donor eyes (age: 31-74 years old) by increasing IOP from 5 to 50mmHg with 0.5mmHg steps below 30mmHg and 2mmHg steps above. The IOP was held constant at each pressure level for 30 seconds. Cross-sectional images of the posterior eye centered on the optic nerve head (ONH) were acquired along the superior-inferior meridian at each pressure step using a 50MHz ultrasound probe (MS700, Vevo2100, VisualSonics). Two Bruch’s membrane termination points were manually delineated in all B-mode images (i.e., at pixel level) (Fig.1). Center and length of the line connecting these two points was tracked at each pressure step to investigate the morphological change of BMO during IOP elevation. Displacements and changes of BMO were calculated relative to the location and diameter of BMO at the baseline pressure (i.e., 5mmHg). Distributive displacement of the posterior eye was calculated by an ultrasound speckle tracking algorithm.

Results : BMO moved posteriorly and the posterior displacement increased with increasing IOP (71.7±51.9µm at 25mmHg and 129.9±82.4µm at 50mmHg) (Fig.2A). Displacement of BMO was larger than the average within ONH at every IOP level (all p<0.001), and highly correlated with the displacement of ONH (R>0.95) (Fig.2B). A negative displacement gradient was observed in the ONH in the through-thickness direction (Fig.2C). The initial BMO at the baseline pressure ranges from 1224.7 to 1609.4µm (1470.2±133.7µm). Change of BMO diameter is minimal in response to IOP elevation (Fig.2D).

Conclusions : We previously reported that there was minimal expansion of the scleral canal (on average about 20 mm) when IOP was raised from 5 to 30mmHg (Ma et al, IOVS, 2019). This study showed minimal change in BMO diameter. BMO moved more posteriorly than the ONH as a whole.

This abstract was presented at the 2019 ARVO Imaging in the Eye Conference, held in Vancouver, Canada, April 26-27, 2019.

 

Fig.1 Delineation of BMO terminations on ultrasound image

Fig.1 Delineation of BMO terminations on ultrasound image

 

Fig.2 (A) Displacement of BMO and ONH (B) correlation of BMO and ONH displacement (C) color map of posterior displacement for a representative human donor eye (D) change of BMO diameter(n=7)

Fig.2 (A) Displacement of BMO and ONH (B) correlation of BMO and ONH displacement (C) color map of posterior displacement for a representative human donor eye (D) change of BMO diameter(n=7)

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×