June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Increased frequency of heat shock protein 27-specific Th1 cells in the peripheral blood of patients with primary open angle glaucoma
Author Affiliations & Notes
  • jing tang
    1. Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
    Ophthalmology department, West China hospital, Chengdu, Sichuan, China
  • Lorenzo Maltish
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Cho Kinsang
    1. Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Julia Devlin
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Rafaella E Silva Nascimento E Silva
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Sherleen Chen
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Eleftherios Paschalis
    1. Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Milica Margeta
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Courtney, Mphil Ondeck
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • David Sola-Del Valle
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • James Chodosh
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Joseph Ciolino
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Roberto Pineda
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Dongfeng Chen
    1. Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Lucy Shen
    Department of Ophthalmology,, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   jing tang, None; Lorenzo Maltish, None; Cho Kinsang, None; Julia Devlin, None; Rafaella Nascimento E Silva, None; Sherleen Chen, None; Eleftherios Paschalis, None; Milica Margeta, None; Courtney, Mphil Ondeck, None; David Sola-Del Valle, None; James Chodosh, None; Joseph Ciolino, None; Roberto Pineda, None; Dongfeng Chen, None; Lucy Shen, None
  • Footnotes
    Support  NIH/NEI EY025259, EY025913 and EY003790, Massachusetts Lions Foundation.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 248. doi:
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      jing tang, Lorenzo Maltish, Cho Kinsang, Julia Devlin, Rafaella E Silva Nascimento E Silva, Sherleen Chen, Eleftherios Paschalis, Milica Margeta, Courtney, Mphil Ondeck, David Sola-Del Valle, James Chodosh, Joseph Ciolino, Roberto Pineda, Dongfeng Chen, Lucy Shen; Increased frequency of heat shock protein 27-specific Th1 cells in the peripheral blood of patients with primary open angle glaucoma. Invest. Ophthalmol. Vis. Sci. 2020;61(7):248.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To identify immunologic biomarkers in patients with primary open angle glaucoma (POAG) with an eventual goal of stratifying different subtypes of glaucoma and predicting disease progression.

Methods : Patients with POAG at the Glaucoma Service at the Mass Eye and Ear (MEE) were classified by disease severity based on Humphrey visual field (VF). Blood samples were collected from POAG patients and control subjects, who were recruited from the Comprehensive Ophthalmology Service at MEE. Patients with any inflammatory disease were excluded from the study. Peripheral bloods were centrifuged to separate the plasma and peripheral blood mononuclear cell (PBMC). PBMCs were stimulated with HSP27 in culture for 2 days, and the frequencies of HSP27-specific cells were detected by flow cytometry. Plasma levels of HSP27, HSP60, TNF-α, IFN-γ, and IL-10 were also assessed by enzyme-linked immunosorbent assay (ELISA) to check for systemic inflammatory status in patients. Statistical significance between groups was determined using one-way ANOVA.

Results : The preliminary results involving 3 POAG patients and 8 controls showed higher frequencies of HSP27-specific Th1 cells (0.2% in POAG patients vs 0.03% in controls, P=0.19), but not other T cell subsets (e.g. Th2, Th17 and Treg), in the PBMC of POAG patients compared to the control subjects. POAG patient with severe VF loss exhibited the highest level of HSP27-specific Th1 cells. No significant differences in plasma levels of HSP27, HSP60, TNF-α, IFN-γ, and IL-10 levels were noted between POAG and control subjects. Strong correlation between the plasma levels of TNF-α (R2=0.99, P=0.01) and body mass index (BMI) in POAG subjects was noted, but not in control groups.

Conclusions : Although recruitment is ongoing, preliminary data suggest potential involvement of HSP27-specific Th1 cells in glaucoma. Frequency of HSP27-specific Th1 cells in PBMC may be linked to disease severity if these results are confirmed in a large cohort of glaucoma patients.

This is a 2020 ARVO Annual Meeting abstract.

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