June 2020
Volume 61, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2020
Association of Aqueous Cytokine Profile and Extracted OCT Features in Diabetic Macular Edema: Assessment of the Anatomic-Biologic Bridge
Author Affiliations & Notes
  • Nicole Cardwell
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Joseph R Abraham
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Charles C. Wykoff
    Retina Consultants of Houston, Texas, United States
  • Leina Lunasco
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • David M Brown
    Retina Consultants of Houston, Texas, United States
  • sruthi arepalli
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Sunil K Srivastava
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Thuy k. Le
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Sarah Biehl
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Carmen Calabrise
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Stephanie Kaiser
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Duriye Damla Sevgi
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Rucha Tadwalkar
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Ming Hu
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Jamie Reese
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Justis P Ehlers
    Cole Eye Institute, Cleveland Clinic, Westerville, Ohio, United States
  • Footnotes
    Commercial Relationships   Nicole Cardwell, None; Joseph Abraham, None; Charles C. Wykoff, Adverum (F), Alimera Sciences (C), Allegro (C), Allergan (C), Alynylam (C), Apellis (C), Bayer (C), Clearside (C), D.O.R.C. (C), EyePoint (C), Genentech/Roch (C), Kodiak (C), Neurotech (F), Notal Vision (C), Novartis (C), ONL Therapeutics (C), Opthea (F), PolyPhotonix (C), RecensMedical (C), Regeneron (C), Regeneron (F), Regenxbio (C), Regenxbio (F), Samsung (F), Santen (C); Leina Lunasco, None; David Brown, Adverum (F), Allergan (F), Allergan (C), Apellis (F), Apellis (C), Bayer (C), Biotime (C), Chengdu Kanghong Biotechonlogy (C), Clearside (F), Gemini (C), Genentech/Roch (F), Genentech/Roch (C), Heidelberg (C), Novartis (F), Novartis (C), OHR (C), Opthea (F), Optos (C), Regeneron (F), Regeneron (C), Regenxbio (C), Samsung (F), Santen (F), Senju (C), Zeiss (C); sruthi arepalli, None; Sunil Srivastava, Allergan (F), Bausch and Lomb (C), Gilead (F), Leica (P), Regeneron (F), Santen (C); Thuy Le, None; Sarah Biehl, None; Carmen Calabrise, None; Stephanie Kaiser, None; Duriye Damla Sevgi, None; Rucha Tadwalkar, None; Ming Hu, None; Jamie Reese, None; Justis Ehlers, Aerpio (F), Aerpio (C), Alcon (F), Alcon (C), Allegro (C), Allergan (F), Allergan (C), Genentech (F), Genentech/Roche (C), Leica (C), Leica (P), Novartis (F), Novartis (C), Regeneron (F), Regeneron (C), Santen (C), Thrombogenics/Oxurion (F), Thrombogenics/Oxurion (C), Zeiss (C)
  • Footnotes
    Support  JPE: RPB Unrestricted Grant to the Cole Eye Institute RPB1508DM. JPE: NIHK23 -EY022947. JPE: Regeneron REGE 1901JE.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 40. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Nicole Cardwell, Joseph R Abraham, Charles C. Wykoff, Leina Lunasco, David M Brown, sruthi arepalli, Sunil K Srivastava, Thuy k. Le, Sarah Biehl, Carmen Calabrise, Stephanie Kaiser, Duriye Damla Sevgi, Rucha Tadwalkar, Ming Hu, Jamie Reese, Justis P Ehlers; Association of Aqueous Cytokine Profile and Extracted OCT Features in Diabetic Macular Edema: Assessment of the Anatomic-Biologic Bridge. Invest. Ophthalmol. Vis. Sci. 2020;61(7):40.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The importance of OCT in the management of diabetic macular edema (DME) is well known. However, there is limited research on potential OCT features that may play a role as biomarkers for the underlying dominant pathogenic mechanism and for predicting treatment response. The purpose of this study is to evaluate the association of specific quantifiable OCT characteristics and the expression of intraocular cytokines.

Methods : The DAVE study is a prospective clinical trial for the management of DME with ranibizumab or ranibizumab combined with targeted panretinal photocoagulation. The IMAGINE study is post-hoc assessment of cytokine expression within the aqueous samples and in-depth assessment of the imaging studies obtained throughout the DAVE study. For this analysis, two multiplex arrays measured levels in quadruplicate for 54 cytokines for each eye. The baseline macular OCT cube was evaluated using an automated multi-layer, feature extraction segmentation platform with expert review and segmentation correction, as needed. Retinal anatomic layers, including ellipsoid zone (EZ) integrity, and volumetric fluid were evaluated. Pearson correlation coefficients assessed the correlation between the measured cytokines and OCT features.

Results : The mean age of 24 included subjects was 54 ± 10, and 19 were from men (79%). Of the 54 cytokines measured, 27 were detected at analyzable levels. Increased panmacular intraretinal fluid volume was directly correlated with LIF (r=0.41, p=0.046) and increased panmacular fluid was correlated with MMP-1 (r=0.44, p=0.030). VEGF did not significantly correlate with fluid volumes or retinal thickness parameters. Multiple cytokines correlated with decreased EZ integrity, as measured by reduced mean central subfield EZ-RPE thickness including uPAR (r= -0.44, p=0.37), HGF (r = -0.57, p=0.0048), LIF (r= -0.47, p=0.022), and MCP-1 (r= -0.46, p=0.025).

Conclusions : Elevated LIF is associated with increased intraretinal fluid volume in treatment-naïve DME, aligning with prior reports of increased LIF levels in diabetic retinopathy. Several cytokines, including MCP-1 and LIF, correlated with decreased ellipsoid zone integrity. Further research is needed to evaluate the potential for OCT-derived biomarkers that may provide insights into the underlying expressed cytokine profile.

This is a 2020 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×