June 2020
Volume 61, Issue 7
ARVO Annual Meeting Abstract  |   June 2020
Effect of different LED characteristics on lethality and reactive oxygen species of M-1 cell and lethality of mouse CD1 brain cortex neuron.
Author Affiliations & Notes
  • Chang Kyu Lee
    ophthalmology department , Ulsan university hospital, Ulsan , Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Chang Kyu Lee, None
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Investigative Ophthalmology & Visual Science June 2020, Vol.61, 652. doi:
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      Chang Kyu Lee; Effect of different LED characteristics on lethality and reactive oxygen species of M-1 cell and lethality of mouse CD1 brain cortex neuron.. Invest. Ophthalmol. Vis. Sci. 2020;61(7):652.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : Retinal ganglion cell(RGC) is the main target tissue of glaucoma and has two distinguishable characters which are mitochondria rich and neuronal cell. Light-emitting diodes(LEDs) could be classified by color rendering index(CRI) and color temperature(CCT). We examined different LED characteristics-induced cell damages in mitochndrial rich cell and neuronal cell.

Methods : We used four different LED classified by CRI and CCT( L1; CRI:98,CCT:3061, L2;CRI:96,CCT:3979, L3;CRI:80,CCT:3855,L4;CRI:76,CCT:4782). M-1 cell was represented as a mitochondria rich cell and CD 1 brain cortex neuron was represented as a neuronal cell. Pathological examinations included water-soluble tetrazolium -1 (WST-1) cell proliferation assays and trypan blue assay. We also measured reactive oxygen species (ROS) in M-1 cell through ROS detection assay kit.

Results : WST-1 and Trypan blue revealed apoptosis of M-1 cells & CD-1 cell under LED lights and amount of apoptosis was significantly different from 2 hours to 24 hours according to different LED color metrics, which meant low CRI and high CCT inducing increased cell apoptosis than high CRI and low CCT. Moreover, ROS assay kit revealed cell apoptosis by LED induced by ROS activation. High CRI and low CCT LED allows cell proliferation rather than apoptosis during certain time period.

Conclusions : Our results showed high quality LED (high CRI and low CCT) emitting more red light than blue light has less toxic effect to M-1 cell and CD-1 cell, which represent RGC’s main character, Therefore, high quality LED may have less toxic effect and more protective effect to RGC.

This is a 2020 ARVO Annual Meeting abstract.


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