June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Proteomic Alterations in the Vitreous of Diabetic Mice
Author Affiliations & Notes
  • Rebekah Robinson
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • Hannah Youngblood
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • TaeJin Lee
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • Justin Bloom
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • Wenbo Zhi
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • Ashok Sharma
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
    Department of Population Health Sciences, Augusta University, Georgia, United States
  • Shruti Sharma
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
    Department of Ophthalmology, Augusta University, Georgia, United States
  • Footnotes
    Commercial Relationships   Rebekah Robinson, None; Hannah Youngblood, None; TaeJin Lee, None; Justin Bloom, None; Wenbo Zhi, None; Ashok Sharma, None; Shruti Sharma, None
  • Footnotes
    Support  NIH/NEI Grant EY026936
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 749. doi:
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      Rebekah Robinson, Hannah Youngblood, TaeJin Lee, Justin Bloom, Wenbo Zhi, Ashok Sharma, Shruti Sharma; Proteomic Alterations in the Vitreous of Diabetic Mice. Invest. Ophthalmol. Vis. Sci. 2020;61(7):749.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Diabetic retinopathy (DR) is the most common complication of diabetes and the number one cause of blindness in the United States. This microvascular disease is caused by prolonged hyperglycemia and characterized by leaky retinal vasculature and ischemia-induced angiogenesis in the retina. Vitreous humor (VH) is a gel-like biofluid in the posterior segment of the eye between the lens and the retina, and because of its location and vascular supply, alterations to the proteins and macromolecules within the retina can also be observed in the VH. Current understanding of VH proteomic changes associated with DR is quite limited; therefore, the purpose of this study was to identify proteomic alterations in VH associated with DR.

Methods : Vitreous samples from control and streptozotocin (STZ)-induced diabetic mice were collected by evisceration. Comprehensive proteomic analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results : We identified a total of 1,367 proteins in the vitreous humor, and the levels of 72 proteins were significantly altered in the mice with DR compared to non-diabetic controls. The proteins demonstrating the greatest change between diabetic and control mice are: Tubulin beta-5 chain (Tubb5), 14-3-3 protein epsilon (Ywhae), Tubulin beta-4B chain (Tubb4b), Gamma-enolase (Eno2), Tubulin beta-2A (Tubb2a), 14-3-3 protein theta (Ywhaq), Lengsin (Lgsn), Glutamine synthetase (Glul), Tubulin alpha-1B chain (Tuba1b), and Rhodopsin (Rho).

Conclusions : LC-MS/MS proteomic characterization of vitreous fluid provides a comprehensive means of analyzing altered protein expression in DR. This offers not only a novel means of studying DR pathology in a controlled environment, but also a critical tool for developing diagnostic biomarkers and an avenue for evaluating future therapeutics.

This is a 2020 ARVO Annual Meeting abstract.

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