Abstract
Purpose :
Chimerine had been shown to be upregulated in serum and vitreous of diabetic patients. the level of cherine also has been shown to be correlated with the severity of diabetic retinopathy in human. However, there is no evidence connecting the expression of its receptors on the proliferative fibrovascular tissue of diabetic patients. In this study we aim to analysis the expression of chemerin receptor on proliferative diabetic retinopathy neovascularization tissue harvested from patients.
Methods :
10 eyes of 10 patients affected by active proliferative diabetic retinopathy were investigated and compared to 8 eyes of 8 patients with no diabetes and epiretinal membrane. The patients had preoperatively received less than two intravitreal antivascular growth factor injection or scant retinal photocoagulations. The patients were undergone parsplana vitrectomy and harvest of either fibrovascular memebrane from proliferative retinopathy diabetic patients or epiretinal memebrane from non diabetic patients serve as control group. The presence and distribution of Chemerin receptor (CMLKR1), CD45 and CD34 was assessed using immunostaining. Chemerin level was assesed in vitreous sample of same patient through enzyme-linked immunosorbent assay.
Results :
The level of human CMKLR1 was significantly higher in membranes of diabetic patients compared to epi-retinal membrane of non-diabetic patients (16% vs 5% of total cell count, P<0.005). The expression of CMKLR1 was both evident within the vascular (CD31 positive) and infiltrating cells (CD45 positive-data cells). Vitreous concentration of chemerine was significantly higher in diabetic group compared to control (5.72ng/ml vs 2.23ng/ml; P<0.005). There was a positive correlation through spearsman analysis between the level of CMKLR1 expression on tissue and CD31 level exprssion (-.0.43; P<0.05).
Conclusions :
Chemerin receptor, CMKLR1 is highly expressed in fibrovascular tissue of diabetic retinopathy patients. This expression is higher in more vascularized memebranes. The expression level may have future therapeutic or prognostic implications.
This is a 2020 ARVO Annual Meeting abstract.