June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Changes of miRNA-15a expression in extracellular vesicles in diabetic retinopathy
Author Affiliations & Notes
  • Tengku Ain Kamalden
    Ophthalmology, University of Malaya Eye Research Centre, Kuala Lumpur, Kuala Lumpur, Malaysia
  • Nurliza Khaliddin
    Ophthalmology, University of Malaya Eye Research Centre, Kuala Lumpur, Kuala Lumpur, Malaysia
  • Nur Musfirah Mahmud
    Ophthalmology, University of Malaya Eye Research Centre, Kuala Lumpur, Kuala Lumpur, Malaysia
  • Adib Redzuan
    University of Malaya, Malaysia
  • Hayatun Syamila Jamil
    Ophthalmology, University of Malaya Eye Research Centre, Kuala Lumpur, Kuala Lumpur, Malaysia
  • Nur Hasyimah Mohd Azemi
    Ophthalmology, University of Malaya Eye Research Centre, Kuala Lumpur, Kuala Lumpur, Malaysia
  • Nadia Hanib
    Ophthalmology, University of Malaya Eye Research Centre, Kuala Lumpur, Kuala Lumpur, Malaysia
  • samarjit das
    Pathology and Anesthesiology, Johns Hopkins School of Medicine, Batlimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Tengku Kamalden, None; Nurliza Khaliddin, None; Nur Musfirah Mahmud, None; Adib Redzuan, None; Hayatun Syamila Jamil, None; Nur Hasyimah Mohd Azemi, None; Nadia Hanib, None; samarjit das, None
  • Footnotes
    Support  Bayer Global Ophthalmology Awards Program, Alcon Research Institute Young Investigator Grant
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 756. doi:
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      Tengku Ain Kamalden, Nurliza Khaliddin, Nur Musfirah Mahmud, Adib Redzuan, Hayatun Syamila Jamil, Nur Hasyimah Mohd Azemi, Nadia Hanib, samarjit das; Changes of miRNA-15a expression in extracellular vesicles in diabetic retinopathy. Invest. Ophthalmol. Vis. Sci. 2020;61(7):756.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To validate isolation methods and investigate miRNA15a expression levels in extracellular vesicles in diabetic retinopathy.

Methods : This cross-sectional study was performed on patients with type 2 Diabetes Mellitus (T2DM) in Kuala Lumpur, Malaysia. Ethical approval was obtained from the University of Malaya Medical Centre Medical Research Ethics Committee. Subjects were divided into 3 groups, namely controls, T2DM with no retinopathy (No DR) and those with non-proliferative diabetic retinopathy (DR). Exclusion criteria included anti-platelets and insulin therapy, smoking history (for controls), anti-VEGF treatment for 6 months, diabetic macular edema and any ocular ischemia.Plasma samples and clinical data were collected. Total circulating miRNA-15a levels were determined using droplet digital PCR (ddPCR) with stem-looped primers. We used miR-451 as a housekeeping gene. Isolation and purification of extracellular vesicles were performed using differential ultracentrifugation technique. Validation of EV was carried out using nanoparticle tracking analysis (ZetaView). MiRNA-15a levels in EVs were determined using real-time PCR.

Results : Samples were collected from 53 controls, 48 in No DR and 18 in DR groups. Age range was 24-77 years old. Using ddPCR, we found that the total plasma miR15-a levels were higher in the diabetics with no retinopathy group compared to controls. This increasing trend was also seen in EVs, although this change was not statistically significant.

Conclusions : We showed that the plasma levels in the T2DM patients with no retinopathy were higher than controls although this was not significant. We found similar increased miR-15a levels in the EVs. Patients with retinopathy (DR) had smaller EVs but in significantly higher concentration compared to controls and No DR. These findings suggest that circulating miR-15a changes occur in T2DM prior to the onset of retinopathy, and may play a role in the early stages of the disease.

This is a 2020 ARVO Annual Meeting abstract.

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