Abstract
Purpose :
We reported previously that enzymatic and singlet oxygen-mediated fatty acid oxidation may be major oxidation pathways in subjects with primary open angle glaucoma (POAG), based on measurement of serum levels of hydroxylinoleate (HODE) and hydroxyarachidonate (HETE) isomers. In this study, we measured serum levels of HODE and HETE isomers in exfoliation syndrome.
Methods :
In total, 311 Japanese subjects comprising EX patients (n = 192) and non-glaucomatous control subjects (n = 119) were included in this study. Patients with EX (n = 192) were divided into EX with glaucoma (EXG) and EX without glaucoma (EXS) groups (n = 128 and n = 64, respectively) depending on the intraocular pressure. After sample reduction and saponification, an aliquot of the sample was analyzed by liquid chromatography/tandem mass spectrometry (LC-MS/MS) on a TSQ Quantum Access Max system (Thermo Fisher Scientific, Waltham, MA, USA) to detect various HODEs, HETEs, and their parent molecules—i.e., linoleic acids and arachidonic acids.
Results :
Total HODE (/linoleic acid) serum levels were significantly (p = 0.0426) higher in the EX group (202.7 ± 153.2 µmol/mol) than in the controls (167.1 ± 105.3 µmol/mol). Among the HODE isomers, the levels of 9-(E,E)-HODEs (p < 0.0001) and 13-(E,E)-HODEs (p < 0.0001), both free radical-mediated oxidation products, were higher in the EX and EXG groups than in the controls, whereas no significant difference was observed between EXS and controls. After adjusting for differences in demographic parameters, multivariate analyses confirmed the association between 9- and 13-(E,E)-HODEs and EX.
Conclusions :
Systemic oxidation was involved in the pathogenesis of EX. Different from the POAG, free radical-mediated oxidationcan be the major pathways.
This is a 2020 ARVO Annual Meeting abstract.