June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Retbindin binds retinoids and regulates sensitivity to light-induced retinal damage
Author Affiliations & Notes
  • Jianguo Fan
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Dinusha Rajapakse
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Katherine Peterson
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Joshua Lerner
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Arjun Ponduri
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Shabnam Parsa
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Vatsala Sagar
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Lijin Dong
    National Eye Institute, Maryland, United States
  • Graeme Wistow
    MSFG, National Eye Institute, Bethesda, Maryland, United States
  • Footnotes
    Commercial Relationships   Jianguo Fan, None; Dinusha Rajapakse, None; Katherine Peterson, None; Joshua Lerner, None; Arjun Ponduri, None; Shabnam Parsa, None; Vatsala Sagar, None; Lijin Dong, None; Graeme Wistow, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 1122. doi:
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      Jianguo Fan, Dinusha Rajapakse, Katherine Peterson, Joshua Lerner, Arjun Ponduri, Shabnam Parsa, Vatsala Sagar, Lijin Dong, Graeme Wistow; Retbindin binds retinoids and regulates sensitivity to light-induced retinal damage. Invest. Ophthalmol. Vis. Sci. 2020;61(7):1122.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Retbindin, a relatively abundant photoreceptor-specific protein, whose function is largely unknown, was investigated to identify its binding partners and to delineate its biological functions in mouse retina.

Methods : Mouse Rtbdn was deleted by classical homologous recombination methods (KO). Visual function of KO mice was measured by ERG and effects of light damage assayed by OCT. Binding activity of recombinant mouse retbindin protein for retinoids, flavonoids and other compounds was measured by bio-layer interferometry (BLI).

Results : Mice with homozygous deletion of Rtbdn (KO), and no expression of any reporter gene, showed no retinal degeneration and normal morphology up to 6 months. Over the same time frame. ERG showed normal amplitudes for light and dark-adapted responses, but a slower implicit time. KO mice on a 129 background were highly resistant to light damage, compared with wild type, suggesting a role involving visual pigments. Older KO mice showed phenotypes resembling premature aging in both inner and other retina. In old KO mice, disc outer segments became disordered, resembling the earlier onset effects of RPE65 deletion. BLI binding assays showed that the recombinant mouse retbindin has significant affinity for retinoids but binds poorly to flavonoids.

Conclusions : Our data suggests retbindin has a role in the visual cycle related to transport of retinoids and is involved in light-induced retinal damage in mice.

This is a 2020 ARVO Annual Meeting abstract.

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