June 2020
Volume 61, Issue 7
ARVO Annual Meeting Abstract  |   June 2020
The cytoskeleton of myotendinous junctions in human extraocular muscles
Author Affiliations & Notes
  • Fatima Pedrosa Domellof
    Dept of Clinical Sciences/Ophthalmology, UMEA University, Umea, Sweden
  • Jing-Xiu Liu
    Integrative Medical Biology, Anatomy, Umeå University, Umeå, Sweden
  • Footnotes
    Commercial Relationships   Fatima Pedrosa Domellof, None; Jing-Xiu Liu, None
  • Footnotes
    Support  Swedish Research Council, Vasterbotten County Council, KMA, Ögonfonden
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 1141. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Fatima Pedrosa Domellof, Jing-Xiu Liu; The cytoskeleton of myotendinous junctions in human extraocular muscles. Invest. Ophthalmol. Vis. Sci. 2020;61(7):1141.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose : To systematically investigate the composition of the cytoskeleton of the myotendinous junctions (MTJs) in human extraocular muscles (EOMs).

Methods : Ten human EOM samples collected with ethical permission were processed for immunofluorescence with antibodies against the cytoskeletal proteins desmin, nestin, vimentin and cytokeratin 19; various myosin heavy chain (MyHC) isoforms as well as antibodies against tenascin or laminin to identify the MTJs.

Results : The majority of the MTJs in both orbital and global layer contained desmin but an important proportion of them did not show increased levels of immunostaining at the folds of the MTJ, in contrast to other muscles. Desmin was absent from approximately 15% of the MTJs and mostly in myofibers containing MyHCIIa. Nestin was present in approximately 91% of the MTJs. Four different combinations were encountered regarding immunolabeling for desmin+nestin at the MTJs, including absence of both in a subgroup of MTJs, irrespective of fiber type. Vimentin was not present at the MTJs and cytokeratin 19 was either present or absent from the MTJs.

Conclusions : The present data on the composition of the cytoskeleton at the MTJs in the EOMs raises fundamental questions regarding our previous knowledge on the role of these proteins for force transmission. We propose a novel model to further investigate these questions.

This is a 2020 ARVO Annual Meeting abstract.


This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.