June 2020
Volume 61, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2020
Cultured human corneal endothelial cells on a collagen vitrigel carrier as a donor tissue for endothelial keratoplasty
Author Affiliations & Notes
  • Wei Wang
    Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Khoa D. Tran
    Lions VisionGift, Oregon, United States
  • Xiaokun Wang
    Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Chris Stoeger
    Lions VisionGift, Oregon, United States
  • Corrina Patzer
    Lions VisionGift, Oregon, United States
  • Jennifer Elisseeff
    Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
    Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, United States
  • Albert S Jun
    Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Wei Wang, Johns Hopkins Technology Ventures, trade secret (P); Khoa Tran, Johns Hopkins Technology Ventures, trade secret (P); Xiaokun Wang, 9,211,256 (P); Chris Stoeger, Johns Hopkins Technology Ventures, trade secret (P); Corrina Patzer, Johns Hopkins Technology Ventures, trade secret (P); Jennifer Elisseeff, 9,211,256 (P); Albert Jun, Johns Hopkins Technology Ventures, trade secret (P)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 1192. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Wei Wang, Khoa D. Tran, Xiaokun Wang, Chris Stoeger, Corrina Patzer, Jennifer Elisseeff, Albert S Jun; Cultured human corneal endothelial cells on a collagen vitrigel carrier as a donor tissue for endothelial keratoplasty. Invest. Ophthalmol. Vis. Sci. 2020;61(7):1192.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The goal of this study was to develop a method to culture corneal endothelial cells onto a transparent collagen vitrigel as a donor tissue for EK.

Methods : Primary human corneal endothelial cells (HCECs) were isolated and expanded in vitro using an animal-free medium. A transparent, thin collagen vitrigel was prepared using a three-step process composed of gelation, vitrification, and rehydration, and the collagen vitrigel was used as a carrier to deliver the cultured HCECs. The HCECs on a 7.5 mm collagen vitrigel was brought into the cat anterior chamber after removing the native endothelium and attached to the posterior stroma (HCEC group). Cat corneas with collagen vitrigel transplantation were used as controls (control group).

Results : Propagated HCECs showed expression of markers indicative of the human corneal endothelium. HCECs cultured on collagen vitrigel exhibited cobblestone-like morphology along with similar/enhanced expression of corneal endothelial markers ZO-1 and Na+/K+-ATPase, compared to identical cultures on plastic tissue culture plate. The cornea was clear in the HCEC group on day 28 after surgery and it was thinning over the 28-day time frame.

Conclusions : This study showed that cell-based therapy for corneal endothelial dysfunction delivered by a collagen vitrigel carrier could be an alternative to traditional corneal transplantation.

This is a 2020 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×