June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Agonistic β2-adrenergic receptor autoantibodies characterize the aqueous humor of patients with primary and secondary open-angle glaucoma
Author Affiliations & Notes
  • Bettina Hohberger
    Department of Ophthalmology, University Erlangen-Nürnberg, Erlangen, Germany
  • Max Wörn
    Department of Ophthalmology, University Erlangen-Nürnberg, Erlangen, Germany
  • Robert Lämmer
    Department of Ophthalmology, University Erlangen-Nürnberg, Erlangen, Germany
  • Martin Herrmann
    Department of Internal Medicine III, Institute of Clinical Immunology and Rheumatology, University of Erlangen-Nürnberg, Germany
  • Christian Y Mardin
    Department of Ophthalmology, University Erlangen-Nürnberg, Erlangen, Germany
  • Friedrich E Kruse
    Department of Ophthalmology, University Erlangen-Nürnberg, Erlangen, Germany
  • Rudolf Kunze
    Science Office, Berlin-Buch, Campus Max Delbrück Center for Molecular Medicine, Germany
  • Gerd Wallukat
    Max Delbrück Center for Molecular Medicine, Germany
  • Footnotes
    Commercial Relationships   Bettina Hohberger, None; Max Wörn, None; Robert Lämmer, None; Martin Herrmann, None; Christian Mardin, None; Friedrich Kruse, None; Rudolf Kunze, None; Gerd Wallukat, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 1222. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Bettina Hohberger, Max Wörn, Robert Lämmer, Martin Herrmann, Christian Y Mardin, Friedrich E Kruse, Rudolf Kunze, Gerd Wallukat; Agonistic β2-adrenergic receptor autoantibodies characterize the aqueous humor of patients with primary and secondary open-angle glaucoma. Invest. Ophthalmol. Vis. Sci. 2020;61(7):1222.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Agonistic β2-adrenergic receptor autoantibodies (β2-AAb) have been detected in serum samples of patients with ocular hypertension (OHT), primary (POAG) and secondary open-angle glaucoma (SOAG), not in healthy controls [1, 2]. It was the aim of the study to investigate the presence of β2-AAb in aqueous humor samples of glaucoma patients in correlation to their β2-AAb serum levels.

1. Jünemann A et al. (2018) Agonistic Autoantibodies to the beta2-Adrenergic Receptor Involved in the Pathogenesis of Open-Angle Glaucoma.
2. Hohberger B et al. (2019) Autoantibodies Activating the beta2-Adrenergic Receptor Characterize Patients With Primary and Secondary Glaucoma.

Methods : 41 patients (22 male, 19 female) were recruited from the Department of Ophthalmology, University of Erlangen-Nürnberg: 22 POAG, 19 SOAG. Aqueous humor (AH) samples were collected during cataract surgery and analyzed for β2-AAb using a cardiomyocyte bioassay. Cut-off was 2 beats / 15 sec. Serum samples of each patient were screened for the presence of β2-AAb by the same method. The study was approved by the local ethics committee and was done in accordance with the tenets of the Declaration of Helsinki. Informed consent was obtained from each participant.

Results : (I) 38 of 41 (92.7%) and 34 of 41 (83.0%) of the patients with OAG showed a β2-AAb in their sera and AH samples, respectively. All patients with β2-AAb in their AH were also seropositive. (II) Subgroup analysis yielded a β2-AAb seropositivity in 95% POAG and 89% SOAG patients. β2-AAb AH-positivity was to be observed in 86% (POAG) and 79% (SOAG). No statistically significant differences were observed for β2-AAb sero- and β2-AAb AH-positivity between glaucoma subgroups (p>0.05). (III) β2-AAb serum levels were not significantly different between POAG (4.7±1.6) and SOAG patients (5.3±1.4; p>0.05). (IV) β2-AAb AH levels were significantly increased in POAG (6.5±1.5) compared to SOAG patients (4.1±1.1; p=0.004). (V) No correlation of β2-AAb AH levels with β2-AAb serum levels was seen for group and subgroup analysis (p>0.05).

Conclusions : The presence of β2-AAb in systemic and local circulation supports the hypothesis of a direct functional impact of these AAb on ocular GPCRs. The high prevalence of β2-AAb in serum and AH of patients with POAG and SOAG argue for a common role in glaucoma pathogenesis, independent of subtype.

This is a 2020 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×