June 2020
Volume 61, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2020
3D OCT-based geometrical changes of human crystalline lenses during simulated accommodation in a lens stretcher
Author Affiliations & Notes
  • Eduardo Martinez-Enriquez
    Visual Optics and Biophotonics Lab, Institute of Optics, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Madrid, Spain
  • Alberto De Castro
    Visual Optics and Biophotonics Lab, Institute of Optics, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Madrid, Spain
  • Ashik Mohamed
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, Telangana, India
    Brien Holden Vision Institute Limited, Sydney, New South Wales, Australia
  • Bianca Maceo Heilman
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Siobhan Williams
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Sushma Nandyala
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, Telangana, India
  • Marco Ruggeri
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Jean-Marie A Parel
    Brien Holden Vision Institute Limited, Sydney, New South Wales, Australia
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Robert Augusteyn
    Brien Holden Vision Institute Limited, Sydney, New South Wales, Australia
    School of Optometry and Vision Science, The University of New South Wales, Sydney, New South Wales, Australia
  • Arthur Ho
    Brien Holden Vision Institute Limited, Sydney, New South Wales, Australia
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Fabrice Manns
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Susana Marcos
    Visual Optics and Biophotonics Lab, Institute of Optics, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Madrid, Spain
  • Footnotes
    Commercial Relationships   Eduardo Martinez-Enriquez, US20170316571A1 (P); Alberto De Castro, None; Ashik Mohamed, None; Bianca Maceo Heilman, None; Siobhan Williams, None; Sushma Nandyala, None; Marco Ruggeri, None; Jean-Marie Parel, None; Robert Augusteyn, None; Arthur Ho, None; Fabrice Manns, None; Susana Marcos, EP15382106 (WO2016142490A1) (P), PCT/ES2012/070185 (P), US20170316571A1 (P)
  • Footnotes
    Support  European Project MyFUN H2020-MSCA-ITN-2015 Ref. 675137, European Project IMCUSTOMEYE H2020-ICT-2017 Ref. 779960​, European Project SILKEYE H2020-ERC-2018-ADG Ref. 833106, European Project COFUND Multiply H-2020-MSCA-COFUND-2015 Ref. 713694, Spanish government grant FIS2017-84753-R, NEI Grants: R01EY021834, F31EY021444 (Maceo), P30EY14801 (Center Grant); the Florida Lions Eye Bank; Research to Prevent Blindness; Drs. KR Olsen and ME Hildebrandt, Drs. Raksha Urs and Aaron Furtado, the Henri and Flore Lesieur Foundation (JMP); Hyderabad Eye Research Foundation.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 1713. doi:
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      Eduardo Martinez-Enriquez, Alberto De Castro, Ashik Mohamed, Bianca Maceo Heilman, Siobhan Williams, Sushma Nandyala, Marco Ruggeri, Jean-Marie A Parel, Robert Augusteyn, Arthur Ho, Fabrice Manns, Susana Marcos; 3D OCT-based geometrical changes of human crystalline lenses during simulated accommodation in a lens stretcher. Invest. Ophthalmol. Vis. Sci. 2020;61(7):1713.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Quantifying human crystalline lens geometry as a function of age and accommodation is essential for understanding disorders such as cataract and presbyopia. We estimated the geometry of the full shape of crystalline lens at different levels of accommodation simulated by a lens stretcher using optical coherence tomography (OCT).

Methods : 15 human donor globes (28-54 y/o) were dissected to preserve lens with its accommodating framework. The tissue was mounted in a motorized miniature lens-stretcher system and immersed in a cuvette filled with BSS. The system simulated disaccommodation by radial stretching of sclera. 3-D OCT images were acquired at 0, 1 and 2 mm of radial displacements, and full shape models were obtained after segmentation of the images and fan and optical distortions correction. For each lens, the average refractive index (n), calculated from the cuvette optical distortion, was considered. Lens volume (VOL), surface area (LSA), thickness (LT), diameter (DIA), radius of curvature of anterior (RAL) and posterior surfaces (RPL), and LT/DIA were quantified. A custom laser ray tracing system coupled with the OCT was used to measure the lens wavefront aberrations and power. Geometrical changes with stretching were analyzed as a function of age using linear regression.

Results : In the maximally accommodated state (0 mm stretching), DIA, VOL, LSA and RAL increased linearly with age at rates of 0.015 mm/yr (r=0.65,p=0.02), 1.55 mm3/yr (r=0.74,p=0.006), 0.93 mm2/yr (r=0.77,p=0.003) and 0.078 mm/yr (r=0.58,p=0.04) respectively. The 2 mm scleral stretch produced changes in lens power that were equivalent to physiological disaccommodation in most of the lenses, ranging from -6.5D (30-35y/o) to 0D (ages above 45y/o). Stretching changes (parameter @2 mm- parameter @0 mm) were higher for younger lenses. Specifically, for ages below 45 y/o:-1.15+0.027yr (r=0.74,p=0.03) for LT, 5.82-0.13yr (r=-0.62,p=0.04) for RAL, and -0.15+0.003yr (r=0.74, p=0.03) for LT/DIA. We did not find significant stretching changes of VOL (mean=0.60±1.90 mm3) or n with age, or of any parameter after 45y/o.

Conclusions : 3-D OCT measurements coupled to a stretcher allow ex vivo quantification of the full shape of the crystalline lens while simulating physiological disaccommodation. Deformations were larger in younger lenses compared to older ones. The lens volume remained constant with stretching at all ages.

This is a 2020 ARVO Annual Meeting abstract.

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