June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Detection of subretinal injection reflux
Author Affiliations & Notes
  • Jin H Shen
    Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, Tennessee, United States
  • Robert Matthews
    Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States
  • Ratna Prasad
    Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, Tennessee, United States
  • Nabil Simaan
    Mechanical Engineering, Computer Science, Otolaryngology, Vanderbilt University, Nashville, Tennessee, United States
  • Karen M Joos
    Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, Tennessee, United States
    Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, United States
  • Footnotes
    Commercial Relationships   Jin Shen, Vanderbilt (P); Robert Matthews, None; Ratna Prasad, None; Nabil Simaan, Vanderbilt (P); Karen Joos, Vanderbilt (P)
  • Footnotes
    Support  NIH/NEI 5R01EY028133-02; Joseph Ellis Family and William Black Research Funds, NEI Core Grant 6P30EY08126 to Vanderbilt Vision Research Center, Unrestricted Departmental Grant from Research to Prevent Blindness, Inc., NY.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 1869. doi:
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    • Get Citation

      Jin H Shen, Robert Matthews, Ratna Prasad, Nabil Simaan, Karen M Joos; Detection of subretinal injection reflux. Invest. Ophthalmol. Vis. Sci. 2020;61(7):1869.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Subretinal injections are increasing in frequency for vitreoretinal gene therapy. We determined whether reflux into the vitreous from the injection could be detected by optical coherence tomography (OCT) imaging.

Methods : A cannula (100 µm OD, 50 µm ID) was introduced through a 23-gauge trocar into the vitreous cavity of fresh ex-vivo porcine eyes. Balanced salt solution (15 µl) containing varying concentrations of microgold (1 μm, Nanopartz, Inc.) was injected subretinally into each eye by a pump (11 Pico Plus Elite programmable syringe pump, Harvard Apparatus). The eyes were then imaged with an 870 nm spectral domain optical coherence tomography (SDOCT) retinal probe (Bioptigen, Inc.).

Results : Subretinal blebs were successfully produced. Hyper-reflective particles within the vitreous cavity emanating from the bleb were detectable with OCT imaging. However, even with full-strength of microgold (1.6E+09 particles/ml), signal was not detected under the subretinal bleb itself. Visualization of the reflux was clearly visible at a dilution of 4E+08 particles/ml, but was rarely detectable at a dilution of 2E+08 particles/ml.

Conclusions : Reflux from subretinal injections labeled with microgold is detectable by 870 nm OCT which may be useful in guiding delivery.

This is a 2020 ARVO Annual Meeting abstract.

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