Abstract
Purpose :
To identify photoreceptor changes in iodoacetic acid induced retinal degeneration feline model
Methods :
From January to October 2014, 11 adult feline (22 eyes) over 2 years of age were included. They divided into 2 groups, 2 for control group and 9 for IAA group. The mean body weight were 1.75 ± 0.35, 1.61 ± 0.19 kg, sex ratio male:female were 1:1, 2:7 respectively. After 12 hours fasting, 20 mg/Kg iodoacetic acid was injected via femoral vein except control group. Before injection and at 1st to 4th week after injection, they had taken ERG (Electroretinogram). Then they were sacrificed and got the enucleation. A standard paraffin section, HE (hematoxylin-eosin) and IHC (Immunohistochemistry) stained retinal section were processed. And histological study including the outer nuclear layer, cone cell and rod cell in photoreceptor was done.
Results :
In IAA group ERG, the mean amplitude of b wave was significantly decreased at 1st to 4th week after injection on both rod(27.43, 29.41, 64.17, 56.03μV) and cone(61.04, 51.25, 131.36, 136.68μV) cell responses compared with baseline(322.48, 610.00μV) examination(p<0.00, 0.00, 0.00, 0.00, 0.00, 0.00, 0.00, 0.00). The optical microscope study showed a significant decrease in the nucleus count of the outer nuclear layer of the retina(16.83±0.89 in control group, 11.98±3.55 in IAA group, p< 0.00), and in the fluorescence microscope study, there was a significant reduction in the mean area per unit length of rod cell layer(35,225.67±2477.02 in control group, 14,903.62±2319.65 in IAA group, p<0.00), but not in cone cell count(26.16±1.34 in control group, 23.98±6.16 in IAA group, p=0.075)
Conclusions :
IAA-induced feline model shows the decrease of b wave amplitude of ERG in functional evaluation, and reduction of outer nuclear layer and rod cells in histological examination. We confirm that IAA-induced feline shows specific photoreceptor degeneration and it can be used further study.
This is a 2020 ARVO Annual Meeting abstract.