June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Myo/Nog cells: A Novel Approach to Neuroprotection in the Retina.
Author Affiliations & Notes
  • Arturo Bravo Nuevo
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Mindy George-Weinstein
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Jacquelyn V Gerhart
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Alexa Noel McGrath
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Samantha Murad
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Paul Lecker
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Karanveer Johal
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • John Spikes
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Jonathan stone
    Anatomy and histology, University of Sydney, Sydney, New South Wales, Australia
  • Alice Brandli
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Diana Crowley
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Rushil Brahmbhatt
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Christopher Sutera
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Jake Bernstein
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Nathan Morrison
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Arturo Bravo Nuevo, None; Mindy George-Weinstein, None; Jacquelyn Gerhart, None; Alexa McGrath, None; Samantha Murad, None; Paul Lecker, None; Karanveer Johal, None; John Spikes, None; Jonathan stone, None; Alice Brandli, None; Diana Crowley, None; Rushil Brahmbhatt, None; Christopher Sutera, None; Jake Bernstein, None; Nathan Morrison, None
  • Footnotes
    Support  Anonymous Foundational Grant
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 2485. doi:
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      Arturo Bravo Nuevo, Mindy George-Weinstein, Jacquelyn V Gerhart, Alexa Noel McGrath, Samantha Murad, Paul Lecker, Karanveer Johal, John Spikes, Jonathan stone, Alice Brandli, Diana Crowley, Rushil Brahmbhatt, Christopher Sutera, Jake Bernstein, Nathan Morrison; Myo/Nog cells: A Novel Approach to Neuroprotection in the Retina.. Invest. Ophthalmol. Vis. Sci. 2020;61(7):2485.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Myo/Nog cells, named for their expression of the skeletal muscle specific transcription factor MyoD and bone morphogenetic protein inhibitor noggin, are critical regulators of embryonic development, including eye morphogenesis. In the normal adult eye, Myo/Nog cells are present in low numbers throughout the anterior and posterior segments. Here we study the behaviors of Myo/Nog cells in different models of retinal stress and degeneration.

Methods : The numbers and distribution of Myo/Nog cells and cell death were determined by immunofluorescence microscopy in the following models: 1) the critical period of physiological culling of photoreceptors, 2) hypoxia induced cell death, 3) light damage, 4) genetically induced retinal degeneration, and 5) increased intraocular pressure. Optical coherence tomography and electroretinography were used to imagine the retina and measure visual function, respectively.

Results : Myo/Nog cells increase in number and migrate to areas of stress and cell death in the retina. Depletion of Myo/Nog cells leads to increased neuronal cell damage. Addition of exogenous Myo/Nog cells significantly reduces neuronal cell death and improves visual function.

Conclusions : Myo/Nog cells are highly responsive to a perturbation in retinal homeostasis induced by different forms of stress and injury. Myo/Nog cells are neuroprotective under these conditions. However, their capacity to differentiate into myofibroblasts must be taken into account when considering an approach of Myo/Nog cell based therapy.

This is a 2020 ARVO Annual Meeting abstract.

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