June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Investigation of effects of decorin on stromal fibrillogenesis in adult un-injured and injured healing cornea using decorin knockout and wild type mice
Sabeeh Kamil; Suneel Gupta; Lynn M. Martin; Ratnakar Tripathi; Nathan Hesemann; Praveen Kumar Balne; Prashant R. Sinha; Nishant Rajiv Sinha; Alexandria Hofmann; Sydney Green; Frederick W Fraunfelder; Rajiv R Mohan
Author Affiliations & Notes
  • Sabeeh Kamil
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Suneel Gupta
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Lynn M. Martin
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Ratnakar Tripathi
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Nathan Hesemann
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Mason Eye Institute, School of Medicine, University of Missouri, Columbia, Missouri, United States
  • Praveen Kumar Balne
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Prashant R. Sinha
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Nishant Rajiv Sinha
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Alexandria Hofmann
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
  • Sydney Green
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
  • Frederick W Fraunfelder
    Mason Eye Institute, School of Medicine, University of Missouri, Columbia, Missouri, United States
  • Rajiv R Mohan
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States
    Harry S. Truman Memorial Veterans’ Hospital, Columbia, Missouri, United States
  • Footnotes
    Commercial Relationships   Sabeeh Kamil, None; Suneel Gupta, None; Lynn Martin, None; Ratnakar Tripathi, None; Nathan Hesemann, None; Praveen Balne, None; Prashant Sinha, None; Nishant Sinha, None; Alexandria Hofmann, None; Sydney Green, None; Frederick Fraunfelder, None; Rajiv Mohan, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 2604. doi:
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      Sabeeh Kamil, Suneel Gupta, Lynn M. Martin, Ratnakar Tripathi, Nathan Hesemann, Praveen Kumar Balne, Prashant R. Sinha, Nishant Rajiv Sinha, Alexandria Hofmann, Sydney Green, Frederick W Fraunfelder, Rajiv R Mohan; Investigation of effects of decorin on stromal fibrillogenesis in adult un-injured and injured healing cornea using decorin knockout and wild type mice. Invest. Ophthalmol. Vis. Sci. 2020;61(7):2604.

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Abstract

Purpose : Collagen fibrils organisation and interaction with small leucine rich proteoglycans (SLRPs) in corneal stroma impact corneal transparency. Gene transfer of decorin, a SLRP, has been previously shown by us to prevent haze in a healing cornea. This study investigated the role of decorin in stromal collagen fibrillogenesis and transparency in adult un-injured and injured-healing mouse cornea using dcn-/- and dcn+/+ transgenic mice.

Methods : Six 10-14 weeks old dcn+/+ and dcn-/- mice of C57BL/6J strain with and without corneal alkali injury were followed for 21 days. Alkali injury was administered on only one cornea of each mouse by placing 2 mm filter paper disc soaked in 0.5 M NaOH for 30 sec. Slit lamp and stereomicroscope were used for clinical ophthalmic examination. Transmission Electron Microscopy (TEM) images of corneal sections, analysed with mathematical image processing tools, were used to study the organisation of collagen fibrils (area and diameter of fibrils, maximum and minimum interfibrillar distance).

Results : Uninjured dcn+/+ and uninjured dcn-/- mouse eyes showed no difference in corneal haze and neovascularisation on clinical examination, and no significant differences in collagen fibril organisation on TEM image analysis. As compared to alkali injured dcn+/+ mouse eyes, alkali injured dcn-/- mouse eyes showed notable corneal haze and neovascularisation on clinical examination. TEM image analysis showed that collagen fibrils with increased diameter and area were found in injured dcn-/- mouse corneas as compared to injured dcn+/+ mouse corneas.

Conclusions : Decorin plays a role in collagen fibrillogenesis and transparency in adult mouse cornea during wound healing in vivo. Ongoing studies using loss-of-function approach are expected to advance the role of decorin in adult healing mouse cornea in vivo.

This is a 2020 ARVO Annual Meeting abstract.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright © 2025 Association for Research in Vision and Ophthalmology.
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