Abstract
Purpose :
To study the antibacterial activity of diacerein eye drops on common pathogenic bacteria on the ocular surface in vitro.
Methods :
ology, including Gram-positive cocci and bacilli, Gram-negative bacilli, filamentous fungi, and Candida. The antibacterial activity was determined in vitro by K-B agar diffusion method, and the minimum inhibitory concentration (MIC) of sensitive Gram-positive cocci (Staphylococcus) was determined by micro-liquid method. The control drug for qualitative and quantitative determination of bacteriostatic activity was levofloxacin. Statistical analysis was performed on the data using an independent sample T test.
Results :
Diacerein eye drops had significant antibacterial activity against 42 strains of Gram-positive cocci isolated from the ocular surface (mainly Staphylococcus), which was not statistically significant compared with levofloxacin (P > 0.05). Diacerein eye drops were also highly sensitive to bacteriostatic activity against 10 strains of Gram-positive bacilli (Corynebacterium) isolated from the ocular surface. The antibacterial activity of the two drugs was not statistically significant (P > 0.05). Diacerein eye drops had no bacteriostatic effect on 23 gram-negative bacilli, 10 filamentous fungi and 3 candida isolated from the ocular surface. The MIC50 of diacerein eye drops on sensitive Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus aureus and other Staphylococcus was 8μg/ml, and those MIC90 was 16, 8, 16, 32μg/ml respectively. MIC value was lower than the corneal concentration of diacetaride.
Conclusions :
Diacerein eye drops have antibacterial activity against Gram-positive bacteria (Staphylococcus and Corynebacterium) isolated from the ocular surface, but have no antibacterial activity against Gram-negative bacilli and fungi. This medicine provides a new drug selection and method for the treatment of bacterial keratitis, but further research is needed for its clinical application.
This is a 2020 ARVO Annual Meeting abstract.