June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
In Vitro Antibacterial Activity of Diacerein on Common Ocular Surface Pathogens
Author Affiliations & Notes
  • Hongmin Zhang
    Henan Provincial People's Hospital of Ophthalmology, Henan Provincial Ophthalmology Hospital, Henan University People's hospital, ZhengZhou, Henan, China
  • Juan Yue
    Henan Provincial People's Hospital of Ophthalmology, Henan Provincial Ophthalmology Hospital, Henan University People's hospital, ZhengZhou, Henan, China
  • Qixue lv
    Henan Provincial People's Hospital of Ophthalmology, Henan Provincial Ophthalmology Hospital, Henan University People's hospital, ZhengZhou, Henan, China
  • Footnotes
    Commercial Relationships   Hongmin Zhang, None; Juan Yue, None; Qixue lv, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 2981. doi:
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      Hongmin Zhang, Juan Yue, Qixue lv; In Vitro Antibacterial Activity of Diacerein on Common Ocular Surface Pathogens. Invest. Ophthalmol. Vis. Sci. 2020;61(7):2981.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To study the antibacterial activity of diacerein eye drops on common pathogenic bacteria on the ocular surface in vitro.

Methods : ology, including Gram-positive cocci and bacilli, Gram-negative bacilli, filamentous fungi, and Candida. The antibacterial activity was determined in vitro by K-B agar diffusion method, and the minimum inhibitory concentration (MIC) of sensitive Gram-positive cocci (Staphylococcus) was determined by micro-liquid method. The control drug for qualitative and quantitative determination of bacteriostatic activity was levofloxacin. Statistical analysis was performed on the data using an independent sample T test.

Results : Diacerein eye drops had significant antibacterial activity against 42 strains of Gram-positive cocci isolated from the ocular surface (mainly Staphylococcus), which was not statistically significant compared with levofloxacin (P > 0.05). Diacerein eye drops were also highly sensitive to bacteriostatic activity against 10 strains of Gram-positive bacilli (Corynebacterium) isolated from the ocular surface. The antibacterial activity of the two drugs was not statistically significant (P > 0.05). Diacerein eye drops had no bacteriostatic effect on 23 gram-negative bacilli, 10 filamentous fungi and 3 candida isolated from the ocular surface. The MIC50 of diacerein eye drops on sensitive Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus aureus and other Staphylococcus was 8μg/ml, and those MIC90 was 16, 8, 16, 32μg/ml respectively. MIC value was lower than the corneal concentration of diacetaride.

Conclusions : Diacerein eye drops have antibacterial activity against Gram-positive bacteria (Staphylococcus and Corynebacterium) isolated from the ocular surface, but have no antibacterial activity against Gram-negative bacilli and fungi. This medicine provides a new drug selection and method for the treatment of bacterial keratitis, but further research is needed for its clinical application.

This is a 2020 ARVO Annual Meeting abstract.

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