Abstract
Purpose :
To evaluate the efficacy of norvancomycin (NVCM) through continuous topical ocular instillation drug delivery (CTOIDD) for treating severe acute experimental keratitis infection with Staphylococcus aureus.
Methods :
Rabbits with experimental keratitis were treated with either NVCM through CTOIDD (experimental group) or topical NVCM eye drop (control group). Clinical signs of keratitis were assessed at days 1, 3, 5, 7 after treatment. Histopathologic examinations were performed to evaluate inflammatory cell infiltration on post-treatment days 7.
Results :
All signs of experimental keratitis were reduced by CTOIDD of NVCM according to clinical criteria, and the experimental group had significantly less inflammation than the control group (p<0.05). 2 rabbits were observed corneal perforation in the control group and none of rabbits showed perforation in the experimental group. Histopathologic examinations showed severe inflammation and marked inflammatory cell infiltration in the control group, but less inflammation in the experimental group (p<0.05).
Conclusions :
Continuous topical ocular instillation delivery of NVCM effectively reduced severe acute inflammation in a rabbit model of severe acute experimental keratitis infection with S.aureus. CTOIDD can be a possible efficient method to manage with the severe acute keratitis.
This is a 2020 ARVO Annual Meeting abstract.