June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Electroretinogram (ERG) recordings unaltered in mice with form deprivation myopia
Author Affiliations & Notes
  • Sreesh Sridhar
    Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States
    Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Healthcare System, Atlanta, Georgia, United States
  • Erica Landis
    Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Healthcare System, Atlanta, Georgia, United States
  • Lauren Hutson
    College of Arts and Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States
  • Machelle Pardue
    Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States
    Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Healthcare System, Atlanta, Georgia, United States
  • Footnotes
    Commercial Relationships   Sreesh Sridhar, None; Erica Landis, None; Lauren Hutson, None; Machelle Pardue, None
  • Footnotes
    Support  NIH R01 EY016435, Dept. of Veterans Affairs Research Career Scientist Award RX003134, Georgia Research Alliance
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 3409. doi:
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    • Get Citation

      Sreesh Sridhar, Erica Landis, Lauren Hutson, Machelle Pardue; Electroretinogram (ERG) recordings unaltered in mice with form deprivation myopia. Invest. Ophthalmol. Vis. Sci. 2020;61(7):3409.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Studies have shown that humans and chickens with myopia have reduced electroretinogram (ERG) amplitudes. Jiang et al. found that mice with lens-induced myopia showed no changes in ERG amplitudes after three weeks of defocus (Sci Rep, 2018). Here, we examined the influence of long-term form deprivation myopia on ERGs in mice.

Methods : Male and female C57BL/6J mice (n=5) were measured for refractive errors and retinal function using dark- and light-adapted ERGs up to 16 weeks postnatal. Mice underwent form deprivation (FD) in the right eye (OD) using head-mounted diffuser goggles at 4 weeks of age. Refractive errors using photorefraction were measured biweekly. Retinal function was assessed after 1, 2, and 3 months of FD. A 2-way, repeated measures ANOVA was used to determine statistical significance.

Results : Myopic shifts developed within 2 weeks of FD (myopic OD - control OS: -3.87 ± 1.84 D) and remained myopic through 3 months of FD (-6.45 ± 1.73 D). Under dark-adapted conditions, myopic eyes were statistically similar in a- and b-wave amplitudes up to 3 months of FD (2.5 log cd s/m2 flash: a-wave OD: -204.51 ± 43.36 µV, OS: -275.43 ± 27.82 µV; b-wave OD: 332.14 ± 84.67 µV, OS: 444.93 ± 40.40 µV). After light-adaptation, myopic and control eyes had a trend for decreased b-wave amplitudes after 3 months of FD, but did not reach significance (2.0 log cd s/m2 flash; b-wave OD: 117.95 ± 26.46 µV, OS: 166.37 ± 19.67 µV). Oscillatory potential parameters and implicit times for all waves showed no significant differences between eyes.

Conclusions : These results suggest myopia may have no noticeable adverse effects on retinal function in mice. In other species, myopic effects on ERG amplitudes have been attributed to axial length elongation. Due to the small ocular size in mice, changes in axial length may not influence the ERG recordings.

This is a 2020 ARVO Annual Meeting abstract.

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