Investigative Ophthalmology & Visual Science Cover Image for Volume 61, Issue 7
June 2020
Volume 61, Issue 7
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ARVO Annual Meeting Abstract  |   June 2020
An in vitro model for reduced ocular rigidity and its effect on intraocular pressure (IOP) fluctuation.
Author Affiliations & Notes
  • Edward R Chu
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Arthur J Sit
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Footnotes
    Commercial Relationships   Edward Chu, None; Arthur Sit, None
  • Footnotes
    Support  Mayo Clinic CCaTS (Center for Clinical and Translational Science) Small Grants Program
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 3431. doi:
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      Edward R Chu, Arthur J Sit; An in vitro model for reduced ocular rigidity and its effect on intraocular pressure (IOP) fluctuation.. Invest. Ophthalmol. Vis. Sci. 2020;61(7):3431.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Ocular rigidity can affect the magnitude of IOP fluctuation in response to a change in intraocular volume. The purpose of this study was to develop an in vitro model of reduced ocular rigidity in porcine eyes, and to determine the effects of ocular rigidity on the degree and duration of IOP elevation due to bolus fluid infusions.

Methods : Six enucleated porcine eyes (3 controls and 3 with reduced ocular rigidity) were used. Reduced ocular rigidity was achieved by placing a circumferential silicone scleral buckle (type 42 band with type 72 sleeve) around the eye. Buckles were initially placed with no tension and eyes were immersed in phosphate buffered saline (PBS) at 35oC. Anterior chambers were cannulated with a 32G needle connected to a 12ml syringe and pump. Eyes were perfused with PBS at 2.5 µL/min until steady state IOP was reached. Buckles were then tightened by 5 mm and again perfused until steady state was reached. A 50µL bolus of PBS was then perfused over 60 seconds, and perfusion rate was returned to 2.5µL/min until return to steady state IOP. This was performed a total of 3 times for each eye. The same procedure was followed for control eyes without placement of the buckle. A pressure transducer (located between the pump and the needle) continuously sampled the IOP data and was recorded with an automated computerized system. Data was exported to Excel and analyzed.

Results : In controls, peak IOP was achieved midway during bolus infusion (average 39 seconds) using approximately 33µL of PBS. In contrast, eyes with reduced rigidity reached peak IOP towards the end of the perfusion (average 59 seconds) using around 49µL. Furthermore, final peak IOP (compared to pre-bolus steady state) is higher in eyes with reduced rigidity (average 16mmHg) compared to controls (average 11mmHg) after 50µL infusion. However, time required to return to steady state IOP after bolus infusion took longer in eyes with reduced rigidity compared to controls (about 2 minutes).

Conclusions : Reduction of ocular rigidity using scleral buckles decreases IOP elevation for smaller infusions of fluid, but higher peak IOP occurred after a 50µL infusion compared to controls. In addition, scleral buckles also demonstrated a delay return to baseline after an IOP spike. Further research is needed to understand effect of scleral buckles and reduced ocular rigidity on IOP fluctuations with different volumes of boluses.

This is a 2020 ARVO Annual Meeting abstract.

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