June 2020
Volume 61, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2020
Viability of Intrastromal Endothelial-Descemet Membrane with Preserved Endothelium Polarity
Author Affiliations & Notes
  • Pietro Rosetta
    Ophthalmology, Humanitas San PioX, Milan, Italy
    Ophthalmology, 5. Humanitas Clinical and Research, Rozano (Mian), Italy
  • Alessandro Ruzza
    2. Fondazione Banca degli Occhi del Veneto, Venice, Italy
  • Mohit Nishitkumar Parekh
    2. Fondazione Banca degli Occhi del Veneto, Venice, Italy
    3.UCL Institute Of Ophthalmology, London, United Kingdom
  • Paola Gallon
    2. Fondazione Banca degli Occhi del Veneto, Venice, Italy
  • Riccardo Vinciguerra
    Ophthalmology, Humanitas San PioX, Milan, Italy
  • Stefano Ferrari
    2. Fondazione Banca degli Occhi del Veneto, Venice, Italy
  • Harminder Dua
    Division of Clinical Neuroscience, 4.Larry A Donoso Laboratory for Eye Research, Academic Section of Ophthalmology, Nottingham, United Kingdom
  • Diego Ponzin
    2. Fondazione Banca degli Occhi del Veneto, Venice, Italy
  • Paolo Vinciguerra
    Ophthalmology, 5. Humanitas Clinical and Research, Rozano (Mian), Italy
    Department of Biomedical Sciences, 6. Humanitas University, Pieve Emanuele (Milan), Italy
  • Footnotes
    Commercial Relationships   Pietro Rosetta, None; Alessandro Ruzza, None; Mohit Parekh, None; Paola Gallon, None; Riccardo Vinciguerra, OCULUS Optikgeräte GmbH (C); Stefano Ferrari, None; Harminder Dua, None; Diego Ponzin, None; Paolo Vinciguerra, OCULUS Optikgeräte GmbH (C)
  • Footnotes
    Support  Obtained funding: Humanitas Clinical and Research Center
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 3600. doi:
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      Pietro Rosetta, Alessandro Ruzza, Mohit Nishitkumar Parekh, Paola Gallon, Riccardo Vinciguerra, Stefano Ferrari, Harminder Dua, Diego Ponzin, Paolo Vinciguerra; Viability of Intrastromal Endothelial-Descemet Membrane with Preserved Endothelium Polarity. Invest. Ophthalmol. Vis. Sci. 2020;61(7):3600.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the viability of a healthy endothelial-Descemet membrane (EDM) graft after the insertion into a stromal pocket of a recipient donor cornea while preserving the polarity of the former

Methods : Research corneas [n=52] were divided into three groups: Group A, where an EDM (obtained from another cornea) with good endothelium was inserted in a stromal pocket endothelium side down; Group B, consisting of control corneas without EDM insertion; and Group C, pre-stripped membranes resting on their stroma used as a second control. The tissues were preserved in tissue culture medium for 21 days at 31°C. Parameters including viability of endothelial cells, expression of tight junction (ZO-1) and thickness were evaluated

Results : After 21 days, all the membranes inserted within the stromal pocket of Group A survived, although an average endothelial cell loss of 30.1% (±18.10) and a mortality of 10.2% (±22.86) were recorded. Qualitative analysis using triple staining with Hoechst, ethidium homodimer and calcein AM confirmed the mortality. ZO-1 was expressed where the cells were present, showing good integrity of tight junctions. Group C showed an average endothelial cell loss of 1.9% (±3.38), a mortality of 0.02% (±0.07) and a higher expression of ZO-1

Conclusions : An EDM graft with endothelium facing downwards can survive in a stromal pocket for at least 3 weeks, with an overall cell mortality of 30%. Further studies will be needed to evaluate the possible outcomes of the insertion of a healthy intrastromal EDMs in edematous corneas

This is a 2020 ARVO Annual Meeting abstract.

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