June 2020
Volume 61, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2020
Characterizing Macrophage-Like Cells in the Living Human Retina using Clinical OCT.
Author Affiliations & Notes
  • Maria Virginia Castanos Toral
    Ophthalmology, New York Eye and Ear Infirmary, Mount Sinai, New York City, New York, United States
  • Davis Zhou
    Ophthalmology, New York Eye and Ear Infirmary, Mount Sinai, New York City, New York, United States
    Icahn School of Medicine at Mount Sinai, New York City, New York, United States
  • Rachel E Linderman
    Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, United States
  • Reilly L. Allison
    Graduate School of Biomedical Science, Medical College of Wisconsin, Milwaukee, Wisconsin, United States
  • Tatyana Milman
    Ophthalmology and Pathology, Wills Eye Hospital and Jefferson University Hospital, Philadelphia, Pennsylvania, United States
  • Joseph Carroll
    Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, United States
    Ophthalmology & Visual Sciences, Medical College of Wisconsin, Milwaukee, Wisconsin, United States
  • Justin V Migacz
    Ophthalmology, New York Eye and Ear Infirmary, Mount Sinai, New York City, New York, United States
  • Richard B Rosen
    Ophthalmology, New York Eye and Ear Infirmary, Mount Sinai, New York City, New York, United States
    Icahn School of Medicine at Mount Sinai, New York City, New York, United States
  • Toco Yuen Ping Chui
    Ophthalmology, New York Eye and Ear Infirmary, Mount Sinai, New York City, New York, United States
    Icahn School of Medicine at Mount Sinai, New York City, New York, United States
  • Footnotes
    Commercial Relationships   Maria Castanos Toral, None; Davis Zhou, None; Rachel Linderman, Optovue (C); Reilly Allison, None; Tatyana Milman, None; Joseph Carroll, AGTC (F), MeiraGTx (C), MeiraGTx (F), Optovue (F), Translational Imaging Innovations (I); Justin Migacz, None; Richard Rosen, Astellas (C), Bayer (C), Boehringer-Ingelheim (C), Diopsys (C), Genentech-Roche (C), GlaucoHealth (I), Guardion Code (I), NanoRetina (C), OD-OS (C), Opticology (I), Optovue (C), Regeneron (C), Teva (C); Toco Chui, None
  • Footnotes
    Support  NH Grant R01EY027301, R01EY024969, P30EY001931
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 4329. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Maria Virginia Castanos Toral, Davis Zhou, Rachel E Linderman, Reilly L. Allison, Tatyana Milman, Joseph Carroll, Justin V Migacz, Richard B Rosen, Toco Yuen Ping Chui; Characterizing Macrophage-Like Cells in the Living Human Retina using Clinical OCT.. Invest. Ophthalmol. Vis. Sci. 2020;61(7):4329.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Retinal macrophages play important roles in immune and defense regulation. Here we examine macrophage-like cells imaged in the living human retina using a clinical OCT system.

Methods : 17 controls and 1 patient each with diabetic retinopathy, retinal vein occlusion, and multiple sclerosis were imaged using a clinical SD-OCT system (Avanti RTVue-XR; Optovue). Ten 3x3mm scans centered at 9° temporal to the fovea and ten 4.5x4.5mm scans at the ONH were obtained and averaged.a In controls, repeatability and reproducibility were measured by imaging the temporal retina at 3 times (twice in one day and 3 days apart). A 3µm OCT-reflectance (OCT-R) slab located above [CJ1] [N2] the ILM surface was used for macrophage-like cell density and nearest neighbor distance (NND) measurements (Fig.A1 & B1). An OCT-angiography (OCT-A) located between the ILM and 48µm below the ILM was overlaid with the respective OCT-R slab (Fig. A2 & B2). Cell density and NND measurements were performed on a 500x500µm ROI near the center of the temporal retina and at the supero- and infero-temporal of the ONH OCT-R images. Axial length was obtained for ocular magnification correction of each eye.


Results : In controls, ramified macrophage-like cells with uniform spatial distribution were visualized on the OCT-R images. Motility was observed between images collected on the same day and those collected 3 days apart (Fig. C). There was considerable individual variation in cell density and NND. Mean±SD cell densities measured at the temporal and ONH were 81±26 cells/mm2 (range: 44-156 cells/mm2) and 59±16 cells/mm2 (range: 32-92 cells/mm2), respectively. Similarly, mean±SD NND measured at the temporal and ONH were 71.5±15.6 µm (range: 52.0-108.8 µm) and 89.5±13.0 µm (range: 62.1-130.5 µm), respectively. Non uniform spatial distribution and altered morphology of the macrophage-like cells were identified in patients with retinopathies.

Conclusions : Our findings showed uniform spatial distribution and morphology of macrophage-like cells on the surface of the ILM with apparent motility over time in healthy controls. Their distribution and morphology suggests an origin of macrophage-like cells such as microglia or hyalocytes.b Further studies should focus on the functions and dynamics of these cells in controls and patients with retinopathies.

This is a 2020 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×