June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Brillouin microscopy of human corneas before and after epi-on cross-linking
Author Affiliations & Notes
  • Peng Shao
    Massachusetts General Hospital, Massachusetts, United States
    Harvard Medical School, Boston, Massachusetts, United States
  • Doyle Stulting
    Woolfson Eye Institute, Georgia, United States
  • Jonathan M. Woolfson
    Woolfson Eye Institute, Georgia, United States
  • Dimitri A. Chernyak
    Intelon Optics, Inc., Lexington, Massachusetts, United States
  • Soek-Hyun Yun
    Harvard Medical School, Boston, Massachusetts, United States
    Massachusetts General Hospital, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Peng Shao, Intelon Optics Inc. (E), Intelon Optics Inc. (P); Doyle Stulting, Avedro (F), Cambrium Medical Technology (F), CorNeat (F), CXL Ophthalmics, LLC (F), EyeYon (F), Intelon Optics Inc (F), Ocuflow (F), Ocumetrics (F), Ophtec (F), Promisight (F); Jonathan M. Woolfson, None; Dimitri A. Chernyak, Intelon Optics Inc. (E); Soek-Hyun Yun, Intelon Optics Inc. (P)
  • Footnotes
    Support  NIH R01EY025454, P41EB015903
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 4709. doi:
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      Peng Shao, Doyle Stulting, Jonathan M. Woolfson, Dimitri A. Chernyak, Soek-Hyun Yun; Brillouin microscopy of human corneas before and after epi-on cross-linking. Invest. Ophthalmol. Vis. Sci. 2020;61(7):4709.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To study the biomechanical effects of epithelium-on crosslinking (CXL)

Methods : It is a prospective observation study conducted at Woolfsen Eye Institute in Atlanta, GA, US. (IRB of Partners HealthCare, the Partners Human Research Committee (PHRC), ChesapeakeIRB (Columbia MD, Pro00023766) and registered with clinicaltrials.gov (NCT03220529). 33 (46 eyes) patients (30±11 yo, F/M: 14/19) with keratoconus undergoing epi-on CXL using riboflavin and UVA light were recruited for the study. Brillouin measurements were obtained preoperatively, and 3 months (n =12 (15 eyes), 31±8 yo, F/M: 6/6) or 6 months (n=14 (15 eyes), 30±10 yo, F/M: 4/10) post-CXL. Forty-one eyes of 26 subjects (38±12 yo, F/M: 16/10) with normal corneas Twenty to forty measurements were obtained to maximize coverage of each cornea. Results were analyzed using descriptive statistics.

Results : A significant increase in the Brillouin shift ( >25 MHz, paired t-test, p<0.005) was seen in the postoperative measurements in the cone region (r < 1 mm form thinnest point) of keratoconic corneas following CXL, suggesting an increase in stiffness as a result of the surgical procedure. Mean Brillouin shift of the whole corneas increase by ~ 20 MHz.

Conclusions : Brillouin microscopy is capable of detecting the corneal stiffening effect soon after a corneal crosslinking procedure that has been shown to increase vision and reduce the corneal curvature of corneas with keratoconus. Brillouin microscopy may provide a direct measurement of corneal stiffness that is induced by CXL. Brillouin technology hold a potential for treatment planning and efficacy evaluation.

This is a 2020 ARVO Annual Meeting abstract.

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