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Minzhong Yu, Ashish Solanki, Stephen Walterhouse, Deepak Nihalani, GLENN LOBO; Catechin protects 661W cells under blue light exposure from Oxidative Stress and ER stress. Invest. Ophthalmol. Vis. Sci. 2020;61(7):4952.
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© ARVO (1962-2015); The Authors (2016-present)
Catechin is a type of antioxidant that is abundant in berries, cocoa, and tea. The current study was to test the protective effect of catechin in 661W photoreceptor cell line from oxidative stress under blue light exposure and to investigate the underlying molecular mechanism of catechin protective effects.
661W cells, the kind gift of Prof. Muayyad Al-Ubaidi, University of Houston, were seeded onto 100-mm cell culture dishes and cultured in DMEM with 10% serum. The dishes of the cells were randomly assigned to a control group, a vehicle group, and 2 treatment groups. After 2 days in culture, the treatment groups were treated with 1 and 2 mM Catechin hydrate (58 mg/ml, Selleckchem.com, Huston, TX, Catalog No.S3974) diluted in dimethyl sulfoxide (DMSO). After 24 hours of the treatment, the cells of the vehicle group and the treatment groups were exposed to blue light (5000 lx) for 2 hours. The blue light was obtained by filtering white fluorescent light by a film filter with a bandpass from between 380 and 570 nm (Midnight Blue 5940, Solar Graphics, Clearwater, FL, USA). After light exposure, the cells were harvested for western blot. Oxidative and ER stress markers, DJ-1, NADPH, and p-IRE1α were analyzed by western blot.
Compared to the control group, the vehicle group after blue light exposure showed marked upregulation of DJ-1, NADPH and p-IRE1α expressions, while the treatment groups with 100 and 200 mM catechin treatment after the blue light exposure did not show marked upregulation of these markers of oxidative stress and ER stress.
Our data showed that catechin can reduce oxidative stress and ER stress in 661W cell line with blue light exposure.
This is a 2020 ARVO Annual Meeting abstract.
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