June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Epigenetic Regulation of Newt Lens Regeneration
Author Affiliations & Notes
  • Aristotle Lucas Kayafas
    Biology, Center for Visual Sciences at Miami University (CVSMU), Miami University, Oxford, Ohio, United States
    Cell, Molecular, Structural Biology Graduate Program, Miami University, Oxford, Ohio, United States
  • Jared Austin Tangeman
    Biology, Center for Visual Sciences at Miami University (CVSMU), Miami University, Oxford, Ohio, United States
    Cell, Molecular, Structural Biology Graduate Program, Miami University, Oxford, Ohio, United States
  • Emily VanZeeland
    Biology, Center for Visual Sciences at Miami University (CVSMU), Miami University, Oxford, Ohio, United States
  • Georgios Tsissios
    Biology, Center for Visual Sciences at Miami University (CVSMU), Miami University, Oxford, Ohio, United States
    Cell, Molecular, Structural Biology Graduate Program, Miami University, Oxford, Ohio, United States
  • Erika Grajales-Esquivel
    Biology, Center for Visual Sciences at Miami University (CVSMU), Miami University, Oxford, Ohio, United States
  • Anthony Sallese
    Biology, Center for Visual Sciences at Miami University (CVSMU), Miami University, Oxford, Ohio, United States
  • Katia Del Rio-Tsonis
    Biology, Center for Visual Sciences at Miami University (CVSMU), Miami University, Oxford, Ohio, United States
  • Footnotes
    Commercial Relationships   Aristotle Kayafas, None; Jared Tangeman, None; Emily VanZeeland, None; Georgios Tsissios, None; Erika Grajales-Esquivel, None; Anthony Sallese, None; Katia Del Rio-Tsonis, None
  • Footnotes
    Support  NEI Grant G02944
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 4993. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Aristotle Lucas Kayafas, Jared Austin Tangeman, Emily VanZeeland, Georgios Tsissios, Erika Grajales-Esquivel, Anthony Sallese, Katia Del Rio-Tsonis; Epigenetic Regulation of Newt Lens Regeneration. Invest. Ophthalmol. Vis. Sci. 2020;61(7):4993.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Following lentectomy the newt is able to regenerate the lens from a subpopulation of iris cells in the dorsal iris, while an identical subpopulation of cells on the ventral iris lacks this regenerative capacity. In this system, ventral iris gene expression is highly plastic and reverts to a more stem/progenitor-like state, allowing for cells to reprogram to replenish the lost lens cells. We hypothesize that a global resetting of the epigenome is required for this regenerative reprogramming to take place.

Methods : Newt dorsal and ventral iris were assessed using RNA-seq to determine expression levels of key genes implicated in epigenetic regulation. Furthermore, DNA methylation marks and histone modifications in the newt iris were measured through immunofluorescence (IF) and 3D reconstruction of reprogramming nuclei. Finally, newt iris Chromatin compaction was measured using quantification of confocal images following lentectomy.

Results : Immunofluorescence of newt iris following lentectomy revealed that during regeneration, the repressive histone mark H3K27Me3 is selectively enriched in the ventral iris. In addition, extensive epigenetic remodeling was observed in the newt iris during regeneration, including acute changes in the DNA modifications 5mC and 5hmC. Analysis of chromatin compaction in newts revealed increased accessibility (i.e. more open chromatin) in dorsal iris when compared to ventral, indicating that increased chromatin accessibility plays a key role in regenerative capacity by moving the regenerating cells towards a plastic, progenitor-like cellular identity.

Conclusions : During regeneration, the dorsal newt iris undergoes marked changes in chromatin organization, particularly through increases in chromatin accessibility and resetting of epigenomic regulatory components. These modifications likely play an indispensable role in the regenerative process through their regulation of key genes.

This is a 2020 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×