Investigative Ophthalmology & Visual Science Cover Image for Volume 61, Issue 7
June 2020
Volume 61, Issue 7
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ARVO Annual Meeting Abstract  |   June 2020
Biomarkers in Human Retinal Pigment Epithelium-derived Extracellular Vesicles: possible signatures of health and disease
Miguel Flores-Bellver; Cui Shi; Jason Mighty; Silvia Aparicio-Domingo; Jing Zhou; Kang Li; Hannah Cobb; Stephen Redenti; Valeria Canto Soler
Author Affiliations & Notes
  • Miguel Flores-Bellver
    Department of Ophthalmology, University of Colorado , Aurora, Colorado, United States
  • Cui Shi
    Department of Biology, City University of New York, Lehman College, New York, New York, United States
  • Jason Mighty
    Department of Biology, City University of New York, Lehman College, New York, New York, United States
  • Silvia Aparicio-Domingo
    Department of Ophthalmology, University of Colorado , Aurora, Colorado, United States
  • Jing Zhou
    Department of Biology, City University of New York, Lehman College, New York, New York, United States
  • Kang Li
    Department of Ophthalmology, University of Colorado , Aurora, Colorado, United States
  • Hannah Cobb
    Department of Ophthalmology, University of Colorado , Aurora, Colorado, United States
  • Stephen Redenti
    Department of Biology, City University of New York, Lehman College, New York, New York, United States
  • Valeria Canto Soler
    Department of Ophthalmology, University of Colorado , Aurora, Colorado, United States
  • Footnotes
    Commercial Relationships   Miguel Flores-Bellver, None; Cui Shi, None; Jason Mighty, None; Silvia Aparicio-Domingo, None; Jing Zhou, None; Kang Li, None; Hannah Cobb, None; Stephen Redenti, None; Valeria Canto Soler, None
  • Footnotes
    Support  BrighFocus Foundation; Research to Prevent Blindness; Challenge Grant to the Department of Ophthalmology, CU; CellSight Development Fund
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 5206. doi:
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      Miguel Flores-Bellver, Cui Shi, Jason Mighty, Silvia Aparicio-Domingo, Jing Zhou, Kang Li, Hannah Cobb, Stephen Redenti, Valeria Canto Soler; Biomarkers in Human Retinal Pigment Epithelium-derived Extracellular Vesicles: possible signatures of health and disease. Invest. Ophthalmol. Vis. Sci. 2020;61(7):5206.

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Abstract

Purpose : The retinal pigment epithelium (RPE) plays an essential physiological role between the photoreceptor cell layer and the vascular network surrounding the eye. This interaction is critical in the maintenance of an internal equilibrium within the retina. Several retinal diseases occur as a consequence of RPE dysfunction. RPE cells derived from human induced pluripotent stem cells (hiPSC) provide a biological system for understanding mechanisms of the physiology of RPE in normal and disease conditions. Our team has established a differentiation protocol that is efficient in producing pure populations of fully differentiated, functionally mature RPE cells from hiPSC. The purpose of this study was to analyze and characterize the population of extracellular vesicles (EVs) released by hiPSC-derived RPE cells. EVs are cell-derived nanoscale particles containing a range of molecular cargo including proteins that could potentially serve as biomarkers of RPE health and disease.

Methods : To characterize EVs secreted by hiPSC-derived RPE cells, we analyzed the release rate, concentration, morphology and proteomic content of EVs obtained from hiPSC-derived RPE at a specific developmental time equivalent to mature functional human RPE.

Results : Proteomic analysis of EVs demonstrated that their cargo includes numerous proteins involved in many different pathways, such as cell communication, signal transduction, cell growth and maintenance, metabolism, cell adhesion and immune response.

Conclusions : This work represents the first proteomic profile from EVs released by hiPSC-derived RPE. EV content provides a snapshot of the originating cell’s phenotype with the potential to serve as a biomarker signature of RPE health and disease. This knowledge could be applied to the potential use of circulating extracellular vesicles as surrogate markers of disease, providing a novel and powerful non-invasive approach.

This is a 2020 ARVO Annual Meeting abstract.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright © 2025 Association for Research in Vision and Ophthalmology.
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