June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
The influence of cataract on fluorescence lifetime imaging ophthalmoscopy (FLIO)
Author Affiliations & Notes
  • Joel-Benjamin Lincke
    University Hospital Inselspital Bern, Bern, Switzerland
  • Chantal Dysli
    University Hospital Inselspital Bern, Bern, Switzerland
  • Damian Jaggi
    University Hospital Inselspital Bern, Bern, Switzerland
  • Rahel Fink
    University of Bern, Switzerland
  • Sebastian Wolf
    University Hospital Inselspital Bern, Bern, Switzerland
  • Martin Sebastian Zinkernagel
    University Hospital Inselspital Bern, Bern, Switzerland
  • Footnotes
    Commercial Relationships   Joel-Benjamin Lincke, Heidelberg Engineering (F); Chantal Dysli, Heidelberg Engineering (F); Damian Jaggi, Heidelberg Engineering (F); Rahel Fink, None; Sebastian Wolf, Allergan (F), Bayer Healthcare Pharmaceuticals (F), Bayer Healthcare Pharmaceuticals (C), Carl Zeiss Meditec (F), Carl Zeiss Meditec (C), Chengdu Kanghong Biotechnology (C), Heidelberg Engineering (F), Heidelberg Engineering (C), Novartis Pharmaceuticals Corporation (F), Novartis Pharmaceuticals Corporation (C), RetinAI (C), Roche (F), Roche (C); Martin Zinkernagel, Allergan (C), Bayer (F), Bayer (C), Heidelberg Engineering (C), Heidelberg Engineering (F), Novartis (C), Novartis (I)
  • Footnotes
    Support  This work was supported by a grant of the Swiss National Science Foundation (SNSF) (#320030_156019)
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 5267. doi:
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    • Get Citation

      Joel-Benjamin Lincke, Chantal Dysli, Damian Jaggi, Rahel Fink, Sebastian Wolf, Martin Sebastian Zinkernagel; The influence of cataract on fluorescence lifetime imaging ophthalmoscopy (FLIO). Invest. Ophthalmol. Vis. Sci. 2020;61(7):5267.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the influence of lens opacifications on fluorescence lifetime imaging ophthalmoscopy (FLIO)

Methods : Nine patients (mean age 70, SD ±8.8 years) before and after cataract surgery were included. For image acquisition, a fluorescence lifetime imaging ophthalmoscope with an excitation wavelength of 470 nm was used. Mean fluorescence lifetimes were recorded in a short (SSC, 498–560 nm), and a long (LSC, 560–720 nm) spectral channel. Retinal autofluorescence lifetimes were measured in subjects with clinically significant cataract before and after cataract surgery. Lens opacification was graded using the LOCS III grading scheme. Additionally, autofluorescence lifetime parameters of the crystalline and the artificial lenses were measured.

Results : The mean fluorescence lifetime (Tm) of the retina decreased significantly after cataract surgery in both spectral channels. We measured 1058 ps (SD±479) versus 386 ps (SD±240) in the SSC (p=0.0019) and 635 ps (SD±240) versus 372 ps (SD±95) in the LSC (p= 0.002). The difference between fluorescence lifetimes before and after cataract surgery was more pronounced in the short spectral channel.

Conclusions : Clinically relevant lens opacification results in significantly longer mean fluorescence lifetimes of the retina. Therefore, the lens status has to be considered when performing cross-sectional fluorescence lifetime analysis. Cataract formation and cataract surgery needs to be taken into account when conducting long time follow-up studies.

This is a 2020 ARVO Annual Meeting abstract.

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