Investigative Ophthalmology & Visual Science Cover Image for Volume 61, Issue 7
June 2020
Volume 61, Issue 7
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ARVO Annual Meeting Abstract  |   June 2020
Exploring the relationship between complex multi-layered choroidal neovascular membranes and choriocapillaris nonperfusion in age-related macular degeneration
Author Affiliations & Notes
  • Peter L Nesper
    Ophthalmology, Northwestern University, Chicago, Illinois, United States
  • Gianna M Dingillo
    Ophthalmology, Northwestern University, Chicago, Illinois, United States
  • Amani A Fawzi
    Ophthalmology, Northwestern University, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Peter Nesper, None; Gianna Dingillo, None; Amani Fawzi, NIH DP3DK108248 (AAF) (F), Optovue, Inc (F)
  • Footnotes
    Support  NIH DP3DK108248 (AAF); Research instrument support was provided by Optovue, Inc., Fremont, California, United States.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 4091. doi:
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    • Get Citation

      Peter L Nesper, Gianna M Dingillo, Amani A Fawzi; Exploring the relationship between complex multi-layered choroidal neovascular membranes and choriocapillaris nonperfusion in age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 2020;61(7):4091.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We sought to explore the relationship between choriocapillaris perfusion and vascular complexity of choroidal neovascular membranes (CNV). We used optical coherence tomography angiography (OCTA) to test the hypothesis that more complex, multi-layered CNV patterns in age-related macular degeneration (AMD) were correlated with reduced blood flow signal in the choriocapillaris.

Methods : We used automated OCTA software (version 2017.1.0.151) to measure choriocapillaris vessel density (%) in the parafoveal sectors outside the CNV region. We also calculated percent choriocapillaris area of nonperfusion (PCAN) outside the CNV (total-PCAN) and within a 200 µm anulus around the CNV (halo-PCAN). We used projection resolved OCTA to assess CNV complexity parameters, including greatest CNV flow height above Bruch’s membrane, number of flow layers and average thickness between flow layers. We used Pearson r tests to compare choriocapillaris perfusion with CNV complexity.

Results : Our initial pilot analysis included 10 of total 40 recruited eyes (9/10 females; mean age 73.9 ± 14.3 years). All were previously treated eyes with a mean of 9.8 ± 12.8 anti-vascular endothelial growth factor injections. We found that greater thickness between CNV flow layers was significantly correlated with reduced choriocapillaris vessel density outside the CNV (r=-0.617; p=0.029). Other CNV complexity parameters showed nonsignificant trends with vessel density and PCAN.

Conclusions : These preliminary results prompt us to complete this study of all 40 eyes, using OCTA to explore the relationship between the choriocapillaris and CNV complexity. The study will be critical in further clarifying the relationship between neovascular AMD lesion complexity and the surrounding choriocapillaris, which could provide important insights regarding vascular perfusion deficits in the choroid around these potentially high flow, complex lesions.

This is a 2020 ARVO Annual Meeting abstract.

 

CNV complexity and choriocapillaris perfusion. A) OCTA B-scan of CNV with red flow overlay showing three CNV flow layers and greatest CNV height. B) OCTA of choriocapillaris with B-scan location (yellow) and location of vessel density (%) measurement in superior (S) and temporal (T) quadrants (blue outline). C) Total-PCAN (green) after removal of CNV region (red). D) 200 µm anulus halo-PCAN (green) after removal of CNV and outer regions (red).

CNV complexity and choriocapillaris perfusion. A) OCTA B-scan of CNV with red flow overlay showing three CNV flow layers and greatest CNV height. B) OCTA of choriocapillaris with B-scan location (yellow) and location of vessel density (%) measurement in superior (S) and temporal (T) quadrants (blue outline). C) Total-PCAN (green) after removal of CNV region (red). D) 200 µm anulus halo-PCAN (green) after removal of CNV and outer regions (red).

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