June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Volumetric SS-OCT imaging of human anterior vitreous structure
Author Affiliations & Notes
  • Alfonso Jimenez-Villar
    Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland
  • Daniel Ruminski
    Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland
  • Raul Duarte-Toledo
    Laboratorio de Óptica, Universidad de Murcia, Murcia, Spain
  • J Sebag
    VMR Institute for Vitreous Macula Retina, Huntington Beach, California, United States
  • Silvestre Manzanera
    Laboratorio de Óptica, Universidad de Murcia, Murcia, Spain
  • Ireneusz Grulkowski
    Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland
  • Pablo Artal
    Laboratorio de Óptica, Universidad de Murcia, Murcia, Spain
  • Footnotes
    Commercial Relationships   Alfonso Jimenez-Villar, None; Daniel Ruminski, None; Raul Duarte-Toledo, None; J Sebag, None; Silvestre Manzanera, None; Ireneusz Grulkowski, None; Pablo Artal, None
  • Footnotes
    Support  Foundation for Polish Science (TEAM Programme, POIR.04.04.00-00-5C9B/17-00), National Science Center (HARMONIA Programme, 2017/26/M/NZ5/00849), European Research Council SEECAT grant (#ERC-2013-AdG-339228), Secretaría de Estado de Investigación, Desarrollo e Innovación (SEIDI; #FIS2016-76163-R), Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia (#19897/GERM/15), and European Regional Development Fund (#EU-FEDER), VMR Research Foundation.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 2548. doi:
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      Alfonso Jimenez-Villar, Daniel Ruminski, Raul Duarte-Toledo, J Sebag, Silvestre Manzanera, Ireneusz Grulkowski, Pablo Artal; Volumetric SS-OCT imaging of human anterior vitreous structure. Invest. Ophthalmol. Vis. Sci. 2020;61(7):2548.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To perform in vivo enhanced three-dimensional visualization of anterior vitreous opacities in subjects of different ages using a swept source OCT (SS-OCT) system.

Methods : This cross-sectional observational study included 100 eyes of 50 volunteers (mean age: 40.0±19.3 yo; age range: 9-77 years). The anterior vitreous behind the crystalline lens was imaged with a prototype SS-OCT operating at 1 μm in an enhanced vitreous imaging mode. In addition, axial length, ocular scattering index, ocular aberrations and contrast sensitivity function were measured for each subject. 3-D volumetric data sets (300×300 A-scans) of the anterior segment and the vitreous covering 8×8 mm2 central area were acquired and registered using Elastix software. Different contrast enhancing approaches were implemented to effectively map vitreous opacities. Static and dynamic vitreous structures were segmented using ImageJ software. The vitreous opacity volume was compared with the corresponding measurements of axial eye length, aberrations, intraocular scattering and contrast sensitivity function (VCTS test).

Results : The high sensitivity of our SS-OCT instrument allowed for visualization of the microstructure and anatomy of the anterior vitreous body. Volumetric data sets generated en-face projection images of vitreous opacities as depth color-coded maps of the vitreous (Fig. 1). The projections and cross-sectional images revealed liquefaction as well as scattering from well-organized opacified structures in the anterior vitreous. The number of hyper-reflective voxels behind the crystalline lens enabled determination of an opacification grade that was correlated with the age (r = 0.8182, P < 0.05).

Conclusions : A custom SS-OCT allows for visualization of in vivo microstructural changes of the vitreous body that were associated with age-related opacification. The proposed method can be used for objective clinical evaluation and management of vitreous-related diseases.

This is a 2020 ARVO Annual Meeting abstract.

 

Figure 1. Projection OCT image of the anterior vitreous. The depth behind the posterior lens interface was color coded.

Figure 1. Projection OCT image of the anterior vitreous. The depth behind the posterior lens interface was color coded.

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