June 2020
Volume 61, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2020
OcuPairTM: A novel dendrimer-hyaluronic acid hydrogel sealant for temporary corneal wound stabilization
Author Affiliations & Notes
  • Siva Pramodh Pramodh Kambhampati
    Opthalmology, Center for Nanomedicine, Wilmer Eye Institute, Johsn Hopkins School of Medicine, Baltimore, Maryland, United States
  • Hui P Lin
    Opthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Rishi Sharma
    Ashvattha Therapeutic. LLC, Baltimore, Maryland, United States
  • Dan Saragnese
    Ashvattha Therapeutic. LLC, Baltimore, Maryland, United States
  • Jeffrey L Cleland
    Ashvattha Therapeutic. LLC, Baltimore, Maryland, United States
  • Samuel C Yiu
    Opthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Kannan Rangaramanujam
    Opthalmology, Center for Nanomedicine, Wilmer Eye Institute, Johsn Hopkins School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Siva Pramodh Kambhampati, Johns Hopkins University (P); Hui Lin, None; Rishi Sharma, Ashvattha Theraeutics LLC (E); Dan Saragnese, Ashvattha Theraeutics LLC (E); Jeffrey Cleland, Ashvattha Theraeutics LLC (C), Ashvattha Therapeutics LLC (I), Ashvattha Therapeutics LLC (E); Samuel Yiu, Johns Hopkins University (P); Kannan Rangaramanujam, Ashvattha Therapeutics LLC (C), Ashvattha Therapeutics LLC (I), Johns Hopkins University (P)
  • Footnotes
    Support  The research is funded by DOD-Temperory Corneal repair: W81XWH-18-C-0180
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 369. doi:
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      Siva Pramodh Pramodh Kambhampati, Hui P Lin, Rishi Sharma, Dan Saragnese, Jeffrey L Cleland, Samuel C Yiu, Kannan Rangaramanujam; OcuPairTM: A novel dendrimer-hyaluronic acid hydrogel sealant for temporary corneal wound stabilization. Invest. Ophthalmol. Vis. Sci. 2020;61(7):369.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Battlefield related traumatic eye injuries (TEI) are one of the leading causes of blindness in military personal. Full-thickness corneal wounds and hypotony are the complications that require immediate attention and temporary stabilization until the military personnel is evacuated to the operating facilities. We have developed a novel, two-component system (OcuPairTM) for temporary corneal wound/globe stabilization.

Methods : The first component is an injectable filler gel comprising of sterile 2% hyaluronic acid. The second component is an adhesive hydrogel comprising of methacrylated hydroxyl dendrimer (D-MA) and methacrylated hyaluronic acid (HA-MA), each of which was synthesized using multi-step synthesis protocol. The sealing capacity of the adhesive hydrogel for different corneal wounds were accessed ex vivo in fresh porcine eyes. The performance of OcuPairTM was evaluated in rabbits with different full-thickness wound architectures (6mm linear, 3.5 mm stellate, 4.5 mm circular and 6mm corneoscleral incisions) (n=12 for each).

Results : At appropriate ratios of HA-MA:D-MA the adhesive hydrogel forms a flexible and transparent sealant within 90 seconds of blue light application. Upon application, the adhesive hydrogel seals the corneal incisions rapidly and withstands increasing IOP >70 mmHg. The transparent OcuPairTM secures different corneal wounds immediately, resulting chamber formation within 24 hrs with good corneal adherence up to 5 days. Clinical assessment indicates the adhesive hydrogel is compatible to cornea and does not inflict any significant signs of inflammation. Cyanoacrylate group, in contrast, demonstrates early dislodgment with significant signs of corneal inflammation.

Conclusions : After the evacuation of the injured military personnel from the warzone, much of the life-saving efforts are focused on treating other injuries. Ocular trauma may be overlooked, resulting in delayed treatment, poor visual outcome or vision loss. OcuPairTM is effective in temporary corneal wound/globe stabilization until more permanent intervention can be applied by the corneal surgeon. Scale-up synthesis and IND enabling studies are underway. Potential trials for temporary corneal wound stabilization following traumatic eye injuries will enable broad application of the device in corneal injuries.

This is a 2020 ARVO Annual Meeting abstract.

 

Ex vivo and in vivo performance of OcuPairTM for temporary corneal/globe stablization

Ex vivo and in vivo performance of OcuPairTM for temporary corneal/globe stablization

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