June 2020
Volume 61, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2020
Focal Loss Analysis of Nerve Fiber Layer Reflectance for Glaucoma Diagnosis
Author Affiliations & Notes
  • Ou Tan
    Ophthalmology, Oregon Health & Science Univ, Portland, Oregon, United States
  • Liang Liu
    Ophthalmology, Oregon Health & Science Univ, Portland, Oregon, United States
  • Qisheng You
    Ophthalmology, Oregon Health & Science Univ, Portland, Oregon, United States
  • Jie Wang
    Ophthalmology, Oregon Health & Science Univ, Portland, Oregon, United States
  • Yali Jia
    Ophthalmology, Oregon Health & Science Univ, Portland, Oregon, United States
  • David Huang
    Ophthalmology, Oregon Health & Science Univ, Portland, Oregon, United States
  • Footnotes
    Commercial Relationships   Ou Tan, Optovue (P); Liang Liu, None; Qisheng You, None; Jie Wang, None; Yali Jia, Optovue (F), Optovue (P); David Huang, Optovue (F), Optovue (I), Optovue (P), Optovue (R)
  • Footnotes
    Support  NIH grants R01 EY023285, R21EY027007 and P30 EY010572, Champalimaud Foundation, and an unrestricted grant from Research to Prevent Blindness to Casey Eye Institute.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 5194. doi:
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    • Get Citation

      Ou Tan, Liang Liu, Qisheng You, Jie Wang, Yali Jia, David Huang; Focal Loss Analysis of Nerve Fiber Layer Reflectance for Glaucoma Diagnosis. Invest. Ophthalmol. Vis. Sci. 2020;61(7):5194.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the focal loss of nerve fiber layer (NFL) reflectance for glaucoma diagnosis.

Methods : Participants were scanned with 4.5x4.5-mm optic disc volumetric scan using a spectral-domain optical coherence tomography (OCT). In each axial line, the OCT reflectivity was summed in NFL, normalized by the average reflectivity in the photoreceptor and pigment epithelium complex, and converted to a logarithm scale. Areas occupied by large retinal vessels were excluded. The normalized NFL reflectance map was processed by an angular frequency filter, to remove high frequency noise, and to correct the directional reflectance bias due to the tilt of the retinal plane. The peripapillay area of the map was divided into 160 superpixels. Low-reflectance superpixels were identified as those with the normalized reflectance below the 5 percentile cutoff in the group of normal subjects (Figure 1). Focal reflectance loss was summed over the low-reflectance superpixels. The diagnostic accuracy of the NFL reflectance parameter was compared to overall NFL thickness using the area under received operative characteristic curve (AROC) and sensitivity at 99% specificity. The normative references for NFL thickness and normalized NFL reflectance were both adjusted for age and axial length.

Results : Thirty-five normal, 65 open-angle glaucoma (30 pre-perimetirc, PPG, and 35 perimetric, PG) participants were enrolled in this study. The focal reflectance loss had significantly higher (p<0.025) diagnostic accuracy (AROC=0.925) than overall average NFL thickness (0.859). Using the 99% specificity cutoff, focal reflectance loss detected 97% of PG eyes and 53% of PPG eyes, which was significantly higher than overall NFL thickness (71% and 23%).

Conclusions : Focal reflectance loss is a novel diagnostic parameter that improved glaucoma diagnostic accuracy compared to NFL thickness.

This is a 2020 ARVO Annual Meeting abstract.

 

Figure 1. Representative OCT reflectance maps from the normal and glaucoma groups. The significance map classifies superpixels into normal, borderline (1-5 percentile among normal population), and abnormal (below 1 percentile among normal population) categories.

Figure 1. Representative OCT reflectance maps from the normal and glaucoma groups. The significance map classifies superpixels into normal, borderline (1-5 percentile among normal population), and abnormal (below 1 percentile among normal population) categories.

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