June 2020
Volume 61, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2020
Visual behavioral deficits correlate with retinal thinning but not cumulative intraocular pressure elevation after intracameral injection of an optically clear hydrogel
Author Affiliations & Notes
  • Jingyuan Zhu
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
    Department of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China
  • Anoop Sainulabdeen
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
  • Jeffrey R Sims
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
  • Muneeb A Faiq
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
  • Hiroshi Ishikawa
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
  • Tianmin Ren
    Department of Ophthalmology, The Chinese University of Hong Kong, Hong Kong, China
  • Yu Yu
    Pleryon Therapeutics Limited, Shenzhen, China
  • Christopher Kai-Shun Leung
    Department of Ophthalmology, The Chinese University of Hong Kong, Hong Kong, China
  • Wenbin Wei
    Department of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China
  • Gadi Wollstein
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
  • Joel S Schuman
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, New York, United States
  • Kevin C Chan
    Department of Ophthalmology, New York University School of Medicine, New York, New York, United States
    Department of Radiology, New York University School of Medicine, NYU Langone Health, New York, New York, United States
  • Footnotes
    Commercial Relationships   Jingyuan Zhu, None; Anoop Sainulabdeen, None; Jeffrey Sims, None; Muneeb Faiq, None; Hiroshi Ishikawa, None; Tianmin Ren, None; Yu Yu, Pleryon Therapeutics Limited (P); Christopher Leung, None; Wenbin Wei, None; Gadi Wollstein, None; Joel Schuman, Zeiss, Inc (P); Kevin Chan, None
  • Footnotes
    Support  This work was supported in part by the National Institutes of Health R01-EY013178 and R01-EY028125 (Bethesda, Maryland); BrightFocus Foundation G2013077. G2016030 and G20190103 (Clarksburg, Maryland); Chinese Government Scholarship for Joint Doctoral Training Program at NYU Langone Health Department of Ophthalmology; Research to Prevent Blindness/Stavros Niarchos Foundation International Research Collaborators Award (New York, New York); and an Unrestricted Grant from Research to Prevent Blindness to NYU Langone Health Department of Ophthalmology.
Investigative Ophthalmology & Visual Science June 2020, Vol.61, 1996. doi:
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    • Get Citation

      Jingyuan Zhu, Anoop Sainulabdeen, Jeffrey R Sims, Muneeb A Faiq, Hiroshi Ishikawa, Tianmin Ren, Yu Yu, Christopher Kai-Shun Leung, Wenbin Wei, Gadi Wollstein, Joel S Schuman, Kevin C Chan; Visual behavioral deficits correlate with retinal thinning but not cumulative intraocular pressure elevation after intracameral injection of an optically clear hydrogel. Invest. Ophthalmol. Vis. Sci. 2020;61(7):1996.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Development and pre-clinical testing of glaucoma neurotherapeutics have been obfuscated by limited experimental models that provide chronic elevation of intraocular pressure (IOP) while preserving optical media clarity for structural and functional assessments over time. In this study, we developed an in vivo model system involving the use of non-invasive tonometry, optical coherence tomography (OCT) and optokinetics to characterize retinal integrity and visual behavior in a novel hydrogel-induced chronic IOP elevation model.

Methods : Six adult C57BL/6J mice underwent unilateral intracameral injection of an optically clear, chemically cross-linked hydrogel composed of hyaluronic acid functionalized with vinyl sulfone and thiol groups. IOP was measured with a rebound tonometer at baseline and 1, 3, 7, 10 and 14 days after hydrogel injection. The optic nerve head (ONH) region was scanned for each eye using OCT at baseline and 2 weeks after injection, and total retinal thickness (TRT) was measured within a 0.26-0.36 mm radius ring centered on the ONH using custom-written software (Fig 1). Visual acuity (VA) was measured for each eye using an optokinetic virtual-reality system at baseline and 2 weeks after injection. Data are presented as mean±SEM.

Results : Intracameral hydrogel injection resulted in mild-to-moderate IOP elevation throughout the 2-week experimental period (Fig 2a). TRT in the hydrogel-injected eye was 10.06±3.61% thinner at 2 weeks post-injection compared to baseline (p<0.01) (Fig 2b). IOP elevation also led to a decline in VA by 58.12±7.22% at 2 weeks post-injection compared to baseline (p<0.001) (Fig 2c). Interestingly, among the hydrogel-injected eyes, cumulative IOP measured from 0 to 14 days post-injection did not correlate with TRT or VA (p>0.05) (Fig 2d-e), whereas TRT was positively associated with VA at 2 weeks post-injection (r=0.824, p<0.05) (Fig 2f). No significant change in IOP, TRT or VA was found in the non-injected eye.

Conclusions : An in vivo glaucoma model system was developed that showed a positive correlation between retinal thinning and visual behavioral deficits after chronic IOP elevation. The weak association between cumulative IOP and TRT or VA suggests additional factors apart from IOP level in contributing to glaucomatous damage after chronic IOP elevation.

This is a 2020 ARVO Annual Meeting abstract.

 

 

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