July 2020
Volume 61, Issue 9
Open Access
ARVO Imaging in the Eye Conference Abstract  |   July 2020
Retinopathy Effects on Distribution of Individual Macrophage-Like Cells Imaged on the Vitreoretinal Interface Using Clinical OCT
Author Affiliations & Notes
  • Richard Rosen
    Ophthalmology, New York Eye and Ear Infirmary, New York, New York, United States
    Ophthalmology, Icahn School of Medicine, New York, New York, United States
  • Maria Castanos Toral
    Ophthalmology, New York Eye and Ear Infirmary, New York, New York, United States
    Ophthalmology, Icahn School of Medicine, New York, New York, United States
  • Davis B. Zhou
    Ophthalmology, New York Eye and Ear Infirmary, New York, New York, United States
    Ophthalmology, Icahn School of Medicine, New York, New York, United States
  • Tatyana Milman
    Opthalmology, Wills Eye Hospital, Philadelphia, Pennsylvania, United States
  • Reilly L. Allison
    Medical College of Wisconsin, Wisconsin, United States
  • Rachel E. Linderman
    Medical College of Wisconsin, Wisconsin, United States
  • Joseph Carroll
    Medical College of Wisconsin, Wisconsin, United States
  • Rishard Weitz
    Ophthalmology, New York Eye and Ear Infirmary, New York, New York, United States
  • Justin V Migacz
    Ophthalmology, New York Eye and Ear Infirmary, New York, New York, United States
    Ophthalmology, Icahn School of Medicine, New York, New York, United States
  • Toco Y.P. Chui
    Ophthalmology, New York Eye and Ear Infirmary, New York, New York, United States
    Ophthalmology, Icahn School of Medicine, New York, New York, United States
  • Footnotes
    Commercial Relationships   Richard Rosen, Astellas (C), Boehringer Ingelheim (C), Cellview (C), NanoRetina (C), Optovue (C); Maria Castanos Toral, None; Davis Zhou, None; Tatyana Milman, None; Reilly Allison, None; Rachel Linderman, None; Joseph Carroll, None; Rishard Weitz, None; Justin Migacz, None; Toco Chui, None
  • Footnotes
    Support  This study was supported by the National Eye Institute of the National Institutes of Health under award numbers R01EY027301, R01EY024969 and P30EY001931. Additional funding for this research was provided by the Marrus Family Foundation, the Wise Family Foundation, the Milbank Foundation, Geraldine Violett Foundation, the New York Eye and Ear Infirmary Research Foundation and the Jorge N. Buxton Microsurgical Foundation.
Investigative Ophthalmology & Visual Science July 2020, Vol.61, PP0028. doi:
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      Richard Rosen, Maria Castanos Toral, Davis B. Zhou, Tatyana Milman, Reilly L. Allison, Rachel E. Linderman, Joseph Carroll, Rishard Weitz, Justin V Migacz, Toco Y.P. Chui; Retinopathy Effects on Distribution of Individual Macrophage-Like Cells Imaged on the Vitreoretinal Interface Using Clinical OCT. Invest. Ophthalmol. Vis. Sci. 2020;61(9):PP0028.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Clinical imaging of retinal macrophage-like cells on the vitreoretinal interface could be an important biomarker of inflammation response in retinal diseases. Here we analyzed distribution and morphology of these cells in a variety of retinal pathologies imaged using a clinical OCT.

Methods : 17 controls, 17 glaucoma patients, 13 patients with various stages of diabetic retinopathy, 9 SCR patients, and 5 RVO patients, were imaged using a clinical SD-OCT system (Avanti RTVue-XR; Optovue). Ten 3x3mm scans centered at 9 ° temporal to the fovea were obtained and averaged.a A 3µm OCT-Reflectance (OCT-R) slab located above the ILM surface was used for macrophage-like cell density and nearest neighbor distance (NND) measurements. Measurements were performed on a 500x500 µm ROI near the center of the OCT-R. In patients, macrophage-like cells arranged in clusters. A 500x500 µm ROI was used to measure macrophage-like cells within these structures. Axial length was obtained for magnification correction.

aPMID:28068370

Results : Ramified macrophage-like cells in control eyes were distributed uniformly over the surface of the ILM. In pathological eyes, cells were ramified but less slender unevenly clustered around targets of activity. In controls, mean±SD cell density was 81±26 cells/mm2 (range: 44-156 cells/mm2) and NND was 71.5±15.6 µm (range: 52.0-108.8 µm), In glaucoma mean±SD cell density was 26.93±20.4 cells/mm2 (range: 6.46 – 47.33) and NND was 156.89±63.84 µm (range: 93.05 – 220.73). In diabetic eyes, mean±SD cell density was 157±53 cells/mm2 (range: 104-224 cells/mm2) and NND was 53.2±10.8 µm (range: 45.2-69 µm). In SCR mean±SD cell density wwas 56±21.66 cells/mm2 (34.34 – 77.66) and NND was 109.40±47.53 µm (61.87 – 156.93). In RVO mean±SD cell density was 84.8±59.62 cells/mm2 (range: 28.18 – 144.42) and NND 160.15±177.64 µm (0 – 337.79).

Conclusions : Distributions of macrophage-like cells were non-uniform in different retinopathies, suggesting migration towards sites of retinal damage. While their exact origin is unknown the ability to study their behavior in vivo using clinical instrumentation offers opportunities for investigating their role in retinal injury and response to therapy.

This is a 2020 Imaging in the Eye Conference abstract.

 

In vivo imaging of macrophage-like cells using clinical OCT. Top row: 3μm OCT-R slab located above the ILM surface. Bottom row: Overlay of OCT-R(green) and respective OCT-A (red) images.

In vivo imaging of macrophage-like cells using clinical OCT. Top row: 3μm OCT-R slab located above the ILM surface. Bottom row: Overlay of OCT-R(green) and respective OCT-A (red) images.

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