Erratum in: “Immunization Against Oxidized Elastin Exacerbates Structural and Functional Damage in Mouse Model of Smoke-Induced Ocular Injury” by Balasubramaniam Annamalai, Crystal Nicholson, Nathaniel Parsons, Sarah Stephenson, Carl Atkinson, Bryan Jones, and Bärbel Rohrer.
Invest Ophthalmol Vis Sci. 2020;61(3):45,
https://doi.org/10.1167/iovs.61.3.45.
After publication of this work, an error was noticed in Figure 8, and a missing reference was identified.
In Figure 8A, the C3α breakdown products were incorrectly labeled. The error did not affect any of the interpretations or conclusions of the article. The original and corrected figures are shown below.
To incorporate the corrections to the labels, changes were made to the following descriptions in the text:
In the Figure 8 legend, in part C, “C3d” was changed to “C3dg/C3d (quantified together due to the close molecular weights of 39 and 34 kDa).” In part D, “C3dg levels” was changed to “C3α′1 levels.” In part E, “C3b levels” was changed to “C3α′ levels,” and “(partial overlap of C3b and C3dg)” was changed to “(partial overlap of C3α′ and C3α′1).”
In the Results section “Immunization with Ox-Elastin Increased Ocular Complement Activation and IgG/IgM Deposition Upon Smoke Exposure,” “the presence of C3 breakdown products C3a, C3b, C3d, and C3dg based on their molecular weights” was changed to “the presence of C3α breakdown products C3a, C3α′, C3α′1, and C3dg based on their molecular weights,” and “all other C3 activation products (C3b, C3d, and C3dg)” was changed to “all other C3 activation products (C3α′, C3α′1, and C3dg/C3d).”
In the Materials and Methods section on Western analysis, there was a space holder for a reference, but it was not inserted. The reference is Thurman JM, Kulik L, Orth H, et al. Detection of complement activation using monoclonal antibodies against C3d. J Clin Invest. 2013;123:2218–2230. It has now been added as Reference 68 and is cited in the text.