June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
EXPOSURE TO ENRICHED ENVIRONMENT PROTECTS THE CENTRAL RETINAL PIGMENT EPITHELIUM AND RETINA ON A DRY-AMD EXPERIMENTAL MODEL IN MICE.
Author Affiliations & Notes
  • Hernan Dieguez
    Department of Human Biochemistry, School of medicine/CEFyBo, UBA/CONICET, Argentina
  • Horacio Eduardo Romeo
    Faculty of Ingeniery and Agrarian Sciences, Pontifical Catholic University of Argentina, BIOMED/UCA/CONICET, Argentina
  • Agustina Alaimo
    Laboratory of cellular Dynamics and NanoTools, IQUIBICEN/CONICET/UBA, Argentina
  • Maria Florencia Gonzalez Fleitas
    Department of Human Biochemistry, School of medicine/CEFyBo, UBA/CONICET, Argentina
  • Pablo Horacio Sande
    Department of Human Biochemistry, School of medicine/CEFyBo, UBA/CONICET, Argentina
  • Ruth Rosenstein
    Department of Human Biochemistry, School of medicine/CEFyBo, UBA/CONICET, Argentina
  • Damian Dorfman
    Department of Human Biochemistry, School of medicine/CEFyBo, UBA/CONICET, Argentina
  • Footnotes
    Commercial Relationships   Hernan Dieguez, None; Horacio Romeo, None; Agustina Alaimo, None; Maria Gonzalez Fleitas, None; Pablo Sande, None; Ruth Rosenstein, None; Damian Dorfman, None
  • Footnotes
    Support  IBRO international travel grant
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 314. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Hernan Dieguez, Horacio Eduardo Romeo, Agustina Alaimo, Maria Florencia Gonzalez Fleitas, Pablo Horacio Sande, Ruth Rosenstein, Damian Dorfman; EXPOSURE TO ENRICHED ENVIRONMENT PROTECTS THE CENTRAL RETINAL PIGMENT EPITHELIUM AND RETINA ON A DRY-AMD EXPERIMENTAL MODEL IN MICE.. Invest. Ophthalmol. Vis. Sci. 2021;62(8):314.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Dry age-related macular degeneration (dAMD), the main blindness cause in the elderly, is characterized by retinal pigment epithelium (RPE) and photoreceptors (PR) atrophy circumscribed to the macular area. At the moment, there are no effective therapies to prevent or delay the vision loss affecting dAMD patients. We have developed a dAMD model induced by superior cervical ganglionectomy (SCGx) in C57BL/6J mice, which reproduces the disease hallmarks exclusively circumscribed to the RPE/outer retina temporal region. Environmental enrichment (EE) is a complex condition that boosts physical and social stimulation. Several reports show that EE provides a better recovery from different neuropathology and retinal alterations. In this context, our aim was analyzing the effect of EE on the alterations induced by experimental dAMD.

Methods : Adult male C57BL/6j mice were submitted to unilateral SCGx, were as the contralateral side was submitted to a sham procedure. Animals were continuously exposed to SE or EE during different intervals. Visual function (electroretinography and visual behavior tests) was analized at 4, 6 and 10 weeks post-SCGx. Retinal histology and ultrastructure were analyzed at 10 weeks post-SCGx. RPE mitocondria and oxidative stress markers were assessed by Western blotting, specific probes, and immunofluorescence at 6 weeks post-SCGx.

Results : SCGx induced a reduction of the melanin content and RPE65 inmunoreactivity at the temporal RPE, as well as a decrease in mitochondria mass, an increase in mitochondria superoxide and lipid peroxidation products. Moreover, SCGx induced ultrastructural alterations at the temporal RPE and PR. EE did not prevent SCGx-induced choroid alterations but significantly prevented the visual dysfunction, histology and ultrastructure alterations, mitochondria and oxidative stress. Moreover, EE reversed the functional and structural damage induced by SCGx when started at 4 weeks post-surgery.

Conclusions : Therefore, exposure to EE prevented, and reversed the damage induced by experimental dAMD, probably through RPE mitochondria protection and RPE oxidative damage prevention, thus becoming as a promising novel therapeutic strategy for dAMD.

This is a 2021 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×