June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Comparison of adaptive optics scanning laser ophthalmoscopy and high-magnification scanning laser ophthalmoscopy
Author Affiliations & Notes
  • Dirk-Uwe G Bartsch
    Ophthalmology, Joan and Irwin Jacobs Retina Center, La Jolla, California, United States
  • Mahima Jhingan
    Ophthalmology, Joan and Irwin Jacobs Retina Center, La Jolla, California, United States
  • Qais Zawaydeh
    Ophthalmology, Joan and Irwin Jacobs Retina Center, La Jolla, California, United States
  • Melina Cavichini Cordeiro
    Ophthalmology, Joan and Irwin Jacobs Retina Center, La Jolla, California, United States
    Ophthalmology, Faculdade de Medicina do ABC, Santo Andre, SP, Brazil
  • William R Freeman
    Ophthalmology, Joan and Irwin Jacobs Retina Center, La Jolla, California, United States
  • Footnotes
    Commercial Relationships   Dirk-Uwe Bartsch, None; Mahima Jhingan, None; Qais Zawaydeh, None; Melina Cavichini Cordeiro, None; William Freeman, None
  • Footnotes
    Support  Supported in part by UCSD Vision Research Center Core Grant P30EY022589, NIH grant R01EY016323 (DUB), and an unrestricted grant from Research to Prevent Blindness, NY (WRF).
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 24. doi:
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    • Get Citation

      Dirk-Uwe G Bartsch, Mahima Jhingan, Qais Zawaydeh, Melina Cavichini Cordeiro, William R Freeman; Comparison of adaptive optics scanning laser ophthalmoscopy and high-magnification scanning laser ophthalmoscopy. Invest. Ophthalmol. Vis. Sci. 2021;62(8):24.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Adaptive-optics scanning laser ophthalmoscopy (AOSLO) uses a wavefront detection and a deformable mirror for wavefront correction. These instruments are very expensive and cumbersome to maintain and operate. We have used the high-magnification module objective for a commercial scanning laser ophthalmoscope to image the photoreceptor cone mosaic. We wanted to compare the photoreceptor images captured with both devices.

Methods :
The Heidelberg Engineering High Magnification Module for the Spectralis SLO is a special lens that images an area of 8° x 8° degrees. The normal imaging modes of high-speed and high-resolution are supported. Blue and green imaging are not supported. We imaged 10 subjects with and without glasses. We used proper refraction to allow imaging with proper spherical and cylindrical correction. We used room light to limit the pupil to about 3 mm to achieve diffraction-limited imaging. We compared the photoreceptor images obtained with the HMM lens with images obtained with a custom-built adaptive optics scanning laser ophthalmoscope (AOSLO).

Results : We found that in more than half the subjects we were able to image the photoreceptor mosaic in the patients using 3 mm pupil and proper spherical and cylindrical correction.
We used image steering to confirm that our imaging areas were similar for the AOSLO and HMM imaging.
The photoreceptor density using the High Magnification Module was within 10% of the density as measured in the same region using the AOSLO.

Conclusions : The High Magnification Module was able to image the photoreceptor cone mosaic in more than half the cases. The use of the HMM lens has some limitations. Since the module is a fixed optical element, the module cannot correct for aberrations. Instead, the HMM uses the diffraction-limited 3 mm pupil to achieve optical imaging conditions. The human eye can be considered diffraction-limited up to a pupil diameter of 3 mm. This was confirmed by our experiments. Increase the pupil diameter beyond 3 mm decreased image quality.
We found that we could use the High Magnification Module for pre-screening of patients for imaging with the AOSLO. It is well known that AOSLO imaging is not possible in all sugjects due to other optical limitations. If we were able to image the mosaic with the HMM lens, it was also possible to image with the AOSLO.

This is a 2021 ARVO Annual Meeting abstract.

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