June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
The RUSH2A study: Baseline microperimetry and SD-OCT measures
Author Affiliations & Notes
  • Eleonora M Lad
    Duke University, Durham, North Carolina, United States
  • Wendi Liang
    Jaeb Centre for Health Research, Tampa, Florida, United States
  • Glenn J Jaffe
    Duke University, Durham, North Carolina, United States
  • Zengtian Deng
    Duke University, Durham, North Carolina, United States
  • Jessica Loo
    Duke University, Durham, North Carolina, United States
  • Dibyendu Mukherjee
    Duke University, Durham, North Carolina, United States
  • Sina Farsiu
    Duke University, Durham, North Carolina, United States
  • Footnotes
    Commercial Relationships   Eleonora Lad, Foundation Fighting Blindness (F), Novartis (F); Wendi Liang, None; Glenn Jaffe, None; Zengtian Deng, None; Jessica Loo, None; Dibyendu Mukherjee, None; Sina Farsiu, Duke University-US patent 8,811,745 (P), Duke University-US patent 9,299,155 (P), Duke University-US patent 9,589,346 (P)
  • Footnotes
    Support  Foundation Fighting Blindness
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2435. doi:
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      Eleonora M Lad, Wendi Liang, Glenn J Jaffe, Zengtian Deng, Jessica Loo, Dibyendu Mukherjee, Sina Farsiu; The RUSH2A study: Baseline microperimetry and SD-OCT measures. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2435.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To study the natural history of retinal degeneration related to disease-causing sequence variants in the USH2A gene, the Foundation Fighting Blindness Consortium is conducting a 4-year multicenter, international natural history study titled Rate of Progression in USH2A-related Retinal Degeneration (RUSH2A). Herein we evaluate baseline mesopic microperimetry (MP) and spectral domain optical coherence tomography (OCT) metrics and their relationships with other baseline characteristics.

Methods : One eye of each patient had MP with a MAIA system and OCT was tested on both eyes with a Heidelberg Spectralis unit. General linear models were used to assess the association between demographic and clinical characteristics, including gender, race, age, disease duration, smoking, use of dietary supplements, MP mean sensitivity, and OCT ellipsoid zone (EZ) area. The association between MP mean sensitivity and OCT EZ with visual acuity (VA), and central subfield thickness (CST within the center 1mm) was assessed using Spearman correlation coefficients.

Results : All study participants (N=127) had OCT, while MP was obtained at selected sites (N=91). Comparing participants with Usher syndrome type 2 (USH2, N=80) to autosomal recessive non-syndromic RP (ARRP, N=47), the central structural measures of OCT EZ (3.1±5.7 mm2 vs 4.3±5.6 mm2, p=0.26) and CST (253.1±57.5 µm vs 263.6 ± 32.9 µm, p=0.26) were similar. Longer disease duration was associated with smaller OCT EZ (p<0.001) and lower mean sensitivity (p=0.01). Comparing MP in USH2 participants (N=55) to those with ARPP (N=36), mean sensitivity was similar by diagnosis (5.4 ± 4.9 dB vs 6.7± 5.1 dB, p=0.22). Point-wise sensitivity from repeated MP tests confirmed that central points (within 10-degree) had better sensitivity than peripheral ones (Figure 1). Better VA, larger OCT EZ area, and larger CST were associated with greater MP mean sensitivity (r>0.3 and p<0.01). Better VA and larger CST were also associated with larger OCT EZ area (r>0.6 and p<0.001).

Conclusions : The baseline RUSH2A data revealed similar OCT and MP metrics between the USH2 and ARRP participants. Longer disease duration was associated with more severe abnormalities in retinal structure and function. MP and OCT measures may be useful to monitor disease progression in future studies of USH2A-mediated disease.

This is a 2021 ARVO Annual Meeting abstract.

 

 

Legend: The red dotted line denotes the 10-central degree. The plot only includes eyes with 3 valid tests

Legend: The red dotted line denotes the 10-central degree. The plot only includes eyes with 3 valid tests

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