June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Fluorescence lifetime imaging ophthalmoscopy (FLIO) follow-up analysis in patients with age-related macular degeneration
Author Affiliations & Notes
  • Jace Buxton
    The University of Utah School of Medicine, Salt Lake City, Utah, United States
  • Lydia Sauer
    John A. Moran Eye Center, Salt Lake City, Utah, United States
  • Alexandra S. Vitale
    John A. Moran Eye Center, Salt Lake City, Utah, United States
  • Natalie K. Modersitzki
    John A. Moran Eye Center, Salt Lake City, Utah, United States
  • Paul S Bernstein
    John A. Moran Eye Center, Salt Lake City, Utah, United States
  • Footnotes
    Commercial Relationships   Jace Buxton, None; Lydia Sauer, Tesseract Health (C); Alexandra Vitale, Tesseract Health (C); Natalie Modersitzki, None; Paul Bernstein, Tesseract Health (C)
  • Footnotes
    Support  NIH grants EY11600 and EY14800; NIH/NEI grant T35 EY026511
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1894. doi:
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    • Get Citation

      Jace Buxton, Lydia Sauer, Alexandra S. Vitale, Natalie K. Modersitzki, Paul S Bernstein; Fluorescence lifetime imaging ophthalmoscopy (FLIO) follow-up analysis in patients with age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1894.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : In eyes with age-related macular degeneration (AMD), fluorescence lifetime imaging ophthalmoscopy (FLIO) shows a characteristic pattern of prolonged mean autofluorescence lifetimes. This study investigates changes in autofluorescence lifetimes over time in patients with AMD to better understand disease progression.

Methods : 26 patients with AMD (mean age 75 ± 9 years) were followed at the Moran Eye Center with a prototype Heidelberg Engineering FLIO. The mean follow-up time was 16 ± 8 months (range 6 - 34 months). Fundus autofluorescence was excited at 473 nm, and FLIO lifetimes were recorded in short (SSC, 498 - 560 nm) and long (LSC, 560 - 720 nm) spectral channels. Mean autofluorescence lifetimes were investigated.

Results : At baseline, FLIO lifetimes in the outer ring (OR) of a standardized early treatment diabetic retinopathy study (ETDRS) grid were 297 ± 54 ps (SSC) and 405 ± 58 ps (LSC). At follow up, the same area showed FLIO lifetimes of 306 ± 59 ps (SSC) and 411 ± 57 ps (LSC), p<0.01 for both spectral channels. The central area (C) did not show any significant changes (P=0.67 and 0.18). The average 12-months prolongation of FLIO lifetimes in the OR was 7 ± 20 ps (SSC) and 7 ± 13 ps (LSC).

Conclusions : In eyes with AMD, prolonged FLIO lifetimes further increase over time, particularly in the OR of the LSC, as eyes progress to more advanced stages of AMD. The rate of progression is variable amongst patients and even between eyes of the same patient. FLIO may be useful to monitor the progression of AMD.

This is a 2021 ARVO Annual Meeting abstract.

 

FLIO lifetimes (LSC) from four different patients with AMD at baseline and follow-up. Individual patients show different progression, independent of age. Progression was classified as either fast, medium, or slow. Follow-up time in months is indicated in the images.

FLIO lifetimes (LSC) from four different patients with AMD at baseline and follow-up. Individual patients show different progression, independent of age. Progression was classified as either fast, medium, or slow. Follow-up time in months is indicated in the images.

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